5-mC antibody

Catalog No. ABIN7540499

Supplier Product No.: 200-301-v02

This Mouse Monoclonal antibody specifically detects in DB, ChIP, ELISA and WB. It exhibits reactivity toward Human.

Quick Overview for 5-mC antibody (ABIN7540499)

Target: 5-mC

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Dot Blot (DB), Chromatin Immunoprecipitation (ChIP), ELISA, Western Blotting (WB)

Clone: 33D3

Product Details

Supplier Product No.: 200-301-v02

Supplier: Rockland

Purpose: 5-mC Antibody

Cross-Reactivity (Details): Cross-reactivity with 5-mC from other sources has not been determined.

Purification: Anti-5-mC Antibody was purified was purified by gel filtration.

Sterility: Sterile filtered

Immunogen: Anti-5-mC Antibody was produced in mice by repeated immunization with 5-methylcytosine.

Isotype: IgG1

Application Details

Application Notes:

ChIP_Dilution: 1 μg/ChIP

ELISA_Dilution: 1:3,000

Western_Blot_Dilution: 1:1,000

Other: Dot Blot: 1:250

Comment:

Anti-5-mC Antibody is tested for Dot Blotting, ELISA, Immunofluorescence, Methylated DNA Immunoprecipitation (MeDIP), and Sequencing (MeDIP-seq). Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: 5-mC

Background: 5-methylcytosine, 5-methyl cytosine,The 5-methylcytosine antibody (clone 33D3) is the most published and widely used antibody for DNA methylation analysis. Anti-5mC is ideal for research in Epigenetics due to its applicability to DNA methylation analysis.