SARS-CoV-2 Spike antibody

Catalog No. ABIN7540517

Supplier Product No.: 200-401-ms9-0.5

This Rabbit Polyclonal antibody specifically detects SARS-CoV-2 Spike in ELISA and WB. It exhibits reactivity toward SARS Coronavirus-2 (SARS-CoV-2).

Quick Overview for SARS-CoV-2 Spike antibody (ABIN7540517)

Target: SARS-CoV-2 Spike

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SARS-CoV-2 Spike antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-SARS-CoV-2 Spike Antibody

Supplier Product No.: 200-401-ms9-0.5

Supplier: Rockland

Purpose: SARS-CoV-2 Whole Spike Protein Antibody

Cross-Reactivity (Details): Anti-SARS-CoV-2 Spike antibody is directed against SARS Coronavirus 2 Spike protein.

Purification: The product was purified from monospecific antiserum by protein A affinity purification.

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified SARS-CoV-2 S protein with His-tag, derived from the transfected human HEK293 cells.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:50,000 - 1:100,000

Western_Blot_Dilution: User Optimized

Comment:

Anti-SARS-CoV-2 Whole Spike protein antibody has been tested for use in ELISA and shows reactivity to SARS COV-2 Whole Spike and COV-2 Spike (S2) protein.  Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 500 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SARS-CoV-2 Spike

Target: SARS-CoV-2 Spike

Alternative Name: SARS-CoV-2 Spike Protein

Background: Rabbit anti-SARS CoV 2 Spike Protein Antibody, Spike glycoprotein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, S glycoprotein, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2),SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) the virus that causes the disease COVID-19, is related to SARS-CoV, MERS-CoV, and other betacoronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive strand RNA virus that consists of several structural proteins including spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein, and other non-structural proteins (NSPs). Spike proteins located on the surface of the virus, have a high affinity for binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial which serves as an entry mechanism for infection. As such, the S protein may be vital to blocking viral infection and vaccine development. Anti-SARS-CoV-2 Spike Protein Antibody is useful for researchers interested in diagnostics, vaccines, and viral research.

UniProt: P0DTC2