POU4F2 antibody (N-Term)

Catalog No. ABIN7540564

Supplier Product No.: 600-101-mj0s

Product Details anti-POU4F2 Antibody

Target: POU4F2 (POU Domain, Class 4, Transcription Factor 2 (POU4F2))

Binding Specificity: N-Term

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This POU4F2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Immunofluorescence (IF)

Supplier Product No.: 600-101-mj0s

Supplier: Rockland

Purpose: Brn3b Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse Brn3b.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Brn3b antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of mouse Pou4f2 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 50,000

Immunohistochemistry_Dilution: 1:100

IF_Microscopy_Dilution: 5 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-Brn3b Antibody has been tested in WB, IHC, and IF.  Expect a band at ~43kDa in western blot using appropriate lysates.  Positive controls used:  Rat pup PND2-6 - minimal cortex brain lysate in WB, Mouse testis in IHC, Post-natal rat pup (PND1) heterogeneous brain cells in IF. 

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-POU4F2 antibody (ABIN7540564)

Mehta, Daghsni, Raeisossadati, Xu, Davis, Naidich, Wang, Tao, Pi, Chen, Kostka, Liu, Gross, Kuwajima, Aldiri: "A cis-regulatory module underlies retinal ganglion cell genesis and axonogenesis." in: Cell reports, Vol. 43, Issue 6, pp. 114291, (2024) (PubMed).

Target Details for POU4F2

Target: POU4F2 (POU Domain, Class 4, Transcription Factor 2 (POU4F2))

Alternative Name: Pou4f2 (POU4F2 Products)

Background: Goat Anti-Brn3b Antibody, Goat Anti-Pou4f2 Antibody, POU domain, class 4, transcription factor 2, Brain-specific homeobox/POU domain protein 3B, Brn-3B, Brain-3B, Brn-3.2,Brn3b (Brain-Specific Homeobox/POU Domain Protein 3B) or POU4F2 (POU Class 4 Homeobox 2) is a DNA-binding transcriptional regulator and coregulator that recognizes and binds to the consensus octamer binding site 5-AT[A/T]A[T/A]T[A/T]A-3 in promoter of target genes. Brn3b plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. It cooperates with the transcription factor ISL1 to achieve RGC fate specification in the developing retina. And plays a role in RGC axon formation and guidance by regulating gene expression of specific target genes. Brn3b plays a role in TNFSF11-mediated terminal osteoclast differentiation. It binds chromatin at promoter region of target genes. It acts either as a transcriptional activator or repressor. It plays a role in the regulation of breast cancer cell growth by promoting transcription activation as well as repression of specific target genes. It is involved in tumor breast progression and invasion. Brn3b also plays a role either as a transcriptional coactivator or corepressor. Anti-Brn3b Antibody is useful for researchers interested in breast cancer research, transcription factor activity, and DNA binding.

Gene ID: 18997

UniProt: Q63934

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Sensory Perception of Sound