PIK3R1 antibody (pTyr607)

Catalog No. ABIN7540641

Supplier Product No.: 600-401-mr1

The Rabbit Polyclonal anti-PIK3R1 antibody has been validated for WB, ELISA, IHC, DB and FM. It is suitable to detect PIK3R1 in samples from Human.

Quick Overview for PIK3R1 antibody (pTyr607) (ABIN7540641)

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB), Fluorescence Microscopy (FM)

Product Details anti-PIK3R1 Antibody

Binding Specificity: pTyr607

Supplier Product No.: 600-401-mr1

Supplier: Rockland

Purpose: PI 3 Kinase p85 alpha phospho Y607 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against the phosphorylated form of human PIK3R1 protein at the pY607 residue. 

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-PI 3 Kinase p85 alpha pY607 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal portion of human PIK3R1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: User Optimized

Other: Dot Blot: 1 μg/mL

Comment:

Anti-PI 3 Kinase p85 alpha pY607 Antibody has been tested in ELISA, DB, and IHC.  Expect a band at ~83.6kDa in western blot using appropriate tissues or lysates.  Positive control used: Human Brain Tissue in Immunohistochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PIK3R1

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Alternative Name: PIK3R1

Background: Rabbit Anti-Phosphoinositide-3-Kinase Regulatory Subunit 1, Phosphatidylinositol 3-Kinase 85 KDa Regulatory Subunit Alpha, Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha), Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha, Phosphoinositide-3-Kinase Regulatory Subunit Alpha, PtdIns-3-Kinase Regulatory Subunit Alpha, PI3K Regulatory Subunit Alpha, PI3-Kinase Subunit P85-Alpha, GRB1, Phosphatidylinositol 3-Kinase, Regulatory Subunit, Polypeptide 1 (P85 Alpha), Phosphatidylinositol 3-Kinase-Associated P-85 Alpha, PtdIns-3-Kinase Regulatory Subunit P85-Alpha, PI3-Kinase Regulatory Subunit Alpha, P85-ALPHA, IMD36, AGM7, P85,PI 3-Kinases (phosphoinositide 3-kinases, PI 3-Ks) are responsible for coordinating a diverse range of cell functions, including proliferation and survival, and plays an important role in the metabolic actions of insulin, a mutation in this gene has been associated with insulin resistance. It binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. It is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Phosphatidylinositol 3-kinase plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. Anti-PI 3 Kinase p85 alpha pY607 Antibody is useful for researchers interested in studying Short Syndrome, Agammaglobulinemia 7 Autosomal Recessive, Gastric Cancer Research, and Interleukin-7 Signaling.

Gene ID: 5295

NCBI Accession: NP_001229395

UniProt: P27986

Pathways: TCR Signaling, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, Warburg Effect