UCHL1 antibody

Catalog No. ABIN7540657

Supplier Product No.: 900-301-e52

Product Details anti-UCHL1 Antibody

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UCHL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Supplier Product No.: 900-301-e52

Supplier: Rockland

Purpose: Ubiquitin Hydrolase (UCHL1) Antibody

Cross-Reactivity (Details): Anti-UCHL1 antibody is directed against human UCHL1 .

Purification: The antibody is protein G purified from concentrated clarified cell culture supernate.

Immunogen: Anti-UCHL1 Monoclonal Antibody was produced in mouse by repeated immunizations with recombinant full length human UCHL1 extensively purified from E. coli.

Clone: BH7

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:5000

Comment:

Anti-Ubiquitin Hydrolase antibody is tested for use in ELISA and Western Blot, IHC, ICC, and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 24 kDa in size corresponding to the UCHL1 proteins in Western blot in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: UCHL1 (UCHL1 Products)

Background: Mouse anti-UCHL1 Antibody, mouse anti-Ubiquitin Hydrolase Antibody, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1,UCHL1 antibody recognizes Ubiquitin C-terminal hydrolase 1 (UCHL1) which is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein. The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice. Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease. Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker. Therefore, UCHL1 monoclonal antibody is ideal for investigators involved in Neuroscience.

Gene ID: 7345

UniProt: P09936

Pathways: Feeding Behaviour