M13 Bacteriophage (N-Term) antibody

Catalog No. ABIN7540664

Supplier Product No.: 600-401-mr5s

This Rabbit Polyclonal antibody specifically detects in ELISA, WB and FM. It exhibits reactivity toward Bacteriophage M13.

Quick Overview for M13 Bacteriophage (N-Term) antibody (ABIN7540664)

Target: M13 Bacteriophage

Reactivity: Bacteriophage M13

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Fluorescence Microscopy (FM)

Product Details

Binding Specificity: N-Term

Supplier Product No.: 600-401-mr5s

Supplier: Rockland

Purpose: Anti-Bacteriophage M13 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against Tail virion protein G7P. 

Purification:  This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-M13 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of Bacteriophage M13 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: User Optimized

Comment:

Anti-Bacteriophage M13 Antibody has been tested in ELISA. Expect a band at ~3.6 kDa in western blot using appropriate lysates or tissues.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: M13 Bacteriophage

Alternative Name: Bacteriophage M13

Background: Tail virion protein G7P, Enterobacteria phage M13, Bacteriophage M13,Coat protein C polypeptide I, Gene 7 protein,Tail virion protein G7P (Bacteriophage M13) may initiate with G9P the virion concomitant assembly-budding process, by interacting with the packaging signal of the viral genome. The assembly-budding takes place at the host inner membrane. In turn, G7P and G9P are present at the end of the filamentous virion that emerges first from the bacterial host. Anti-Bacteriophage M13 Antibody is useful for researchers interested in HCP, Host Cell Membranes, Transmembrane Viruses, and E. coli bacteria.

UniProt: P69535