SARS-CoV-2 Spike antibody (RBD)

Catalog No. ABIN7540677

Supplier Product No.: 600-401-ms8

The Rabbit Polyclonal anti-SARS-CoV-2 Spike antibody has been validated for ELISA, IF and FM. It is suitable to detect SARS-CoV-2 Spike in samples from SARS Coronavirus-2 (SARS-CoV-2). There are 3+ publications available.

Quick Overview for SARS-CoV-2 Spike antibody (RBD) (ABIN7540677)

Target: SARS-CoV-2 Spike

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SARS-CoV-2 Spike antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-SARS-CoV-2 Spike Antibody

Binding Specificity: RBD

Supplier Product No.: 600-401-ms8

Supplier: Rockland

Purpose: SARS-CoV-2 Spike Protein (RBD) Antibody

Cross-Reactivity (Details): Anti-SARS-CoV-2 Spike affinity purified antibody is directed against SARS Coronavirus 2 Spike protein.

Purification: The product was purified from monospecific antiserum by immunoaffinity chromatography over SARS CoV-2 Spike resin.

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the SARS Coronavirus Spike Protein within the Receptor Binding Domain (RBD).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:150,000 - 1:450,000

IF_Microscopy_Dilution: 1:1000

Comment:

Suggested Applications: Other
Anti-SARS-CoV-2 Spike protein purified antibody has been tested for use in ELISA and IF. This antibody shows reactivity to recombinant SARS COV-2, S1 subunit protein and recombinant SARS COV-2 whole spike protein.  Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-SARS-CoV-2 Spike antibody (ABIN7540677)

Kaleta, Kern, Hong, Hölzer, Kochs, Beer, Schnepf, Schwemmle, Bollen, Kolb, Huber, Ulferts, Weigang, Dudas, Wittig, Jaki, Padane, Lagare, Salou, Ozer, Nnaemeka, Odoom, Rutayisire, Benkahla, Akoua-Koffi, Ouedraogo, Simon-Lorière, Enouf, Kröger, Calvignac-Sp: "Antibody escape and global spread of SARS-CoV-2 lineage A.27." in: Nature communications, Vol. 13, Issue 1, pp. 1152, (2022) (PubMed).

Weigang, Fuchs, Zimmer, Schnepf, Kern, Beer, Luxenburger, Ankerhold, Falcone, Kemming, Hofmann, Thimme, Neumann-Haefelin, Ulferts, Grosse, Hornuss, Tanriver, Rieg, Wagner, Huzly, Schwemmle, Panning, Kochs: "Within-host evolution of SARS-CoV-2 in an immunosuppressed COVID-19 patient as a source of immune escape variants." in: Nature communications, Vol. 12, Issue 1, pp. 6405, (2021) (PubMed).

Wang, Wang, Gupta, Morrissey, Naik, Singamaneni: "Enhancing the Stability of COVID-19 Serological Assay through Metal-Organic Framework Encapsulation." in: Advanced healthcare materials, Vol. 10, Issue 18, pp. e2100410, (2021) (PubMed).

Target Details for SARS-CoV-2 Spike

Target: SARS-CoV-2 Spike

Alternative Name: SARS-CoV-2 Spike Protein

Background: Rabbit anti-SARS CoV 2 Spike Protein Receptor Binding Domain Antibody, Spike glycoprotein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, S glycoprotein, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2),SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) the virus that causes the disease COVID-19, is related to SARS-CoV, MERS-CoV, and other betacoronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive strand RNA virus that consists of several structural proteins including spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein, and other non-structural proteins (NSPs). Spike proteins located on the surface of the virus, have a high affinity for binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial which serves as an entry mechanism for infection. As such, the S protein may be vital to blocking viral infection and vaccine development. Anti-SARS-CoV-2 Spike Protein Antibody is useful for researchers interested in diagnostics, vaccines, and viral research.

UniProt: P0DTC2