SARS-CoV-2 Spike antibody (RBD)

Catalog No. ABIN7540678

Supplier Product No.: 600-401-ms8s

This anti-SARS-CoV-2 Spike antibody is a Rabbit Polyclonal antibody detecting SARS-CoV-2 Spike in ELISA, IF and FM. Suitable for SARS Coronavirus-2 (SARS-CoV-2). This Primary Antibody has been cited in 3+ publications.

Quick Overview for SARS-CoV-2 Spike antibody (RBD) (ABIN7540678)

Target: SARS-CoV-2 Spike

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SARS-CoV-2 Spike antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-SARS-CoV-2 Spike Antibody

Binding Specificity: RBD

Supplier Product No.: 600-401-ms8s

Supplier: Rockland

Purpose: SARS-CoV-2 Spike Protein (RBD) Antibody

Cross-Reactivity (Details): Anti-SARS-CoV-2 Spike affinity purified antibody is directed against SARS Coronavirus 2 Spike protein.

Purification: The product was purified from monospecific antiserum by immunoaffinity chromatography over SARS CoV-2 Spike resin.

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the SARS Coronavirus Spike Protein within the Receptor Binding Domain (RBD).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:150,000 - 1:450,000

IF_Microscopy_Dilution: 1:1000

Comment:

Suggested Applications: Other
Anti-SARS-CoV-2 Spike protein purified antibody has been tested for use in ELISA and IF. This antibody shows reactivity to recombinant SARS COV-2, S1 subunit protein and recombinant SARS COV-2 whole spike protein.  Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-SARS-CoV-2 Spike antibody (ABIN7540678)

Kaleta, Kern, Hong, Hölzer, Kochs, Beer, Schnepf, Schwemmle, Bollen, Kolb, Huber, Ulferts, Weigang, Dudas, Wittig, Jaki, Padane, Lagare, Salou, Ozer, Nnaemeka, Odoom, Rutayisire, Benkahla, Akoua-Koffi, Ouedraogo, Simon-Lorière, Enouf, Kröger, Calvignac-Sp: "Antibody escape and global spread of SARS-CoV-2 lineage A.27." in: Nature communications, Vol. 13, Issue 1, pp. 1152, (2022) (PubMed).

Weigang, Fuchs, Zimmer, Schnepf, Kern, Beer, Luxenburger, Ankerhold, Falcone, Kemming, Hofmann, Thimme, Neumann-Haefelin, Ulferts, Grosse, Hornuss, Tanriver, Rieg, Wagner, Huzly, Schwemmle, Panning, Kochs: "Within-host evolution of SARS-CoV-2 in an immunosuppressed COVID-19 patient as a source of immune escape variants." in: Nature communications, Vol. 12, Issue 1, pp. 6405, (2021) (PubMed).

Wang, Wang, Gupta, Morrissey, Naik, Singamaneni: "Enhancing the Stability of COVID-19 Serological Assay through Metal-Organic Framework Encapsulation." in: Advanced healthcare materials, Vol. 10, Issue 18, pp. e2100410, (2021) (PubMed).

Target Details for SARS-CoV-2 Spike

Target: SARS-CoV-2 Spike

Alternative Name: SARS-CoV-2 Spike Protein

Background: Rabbit anti-SARS CoV 2 Spike Protein Receptor Binding Domain Antibody, Spike glycoprotein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, S glycoprotein, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2),SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) the virus that causes the disease COVID-19, is related to SARS-CoV, MERS-CoV, and other betacoronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive strand RNA virus that consists of several structural proteins including spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein, and other non-structural proteins (NSPs). Spike proteins located on the surface of the virus, have a high affinity for binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial which serves as an entry mechanism for infection. As such, the S protein may be vital to blocking viral infection and vaccine development. Anti-SARS-CoV-2 Spike Protein Antibody is useful for researchers interested in diagnostics, vaccines, and viral research.

UniProt: P0DTC2