Recombinant Fc gamma RII (CD32) antibody

Catalog No. ABIN7542954

The Rat Chimeric anti- antibody has been validated for FACS, WB, IHC, IF, St and BP. It is suitable to detect in samples from Mouse.

Quick Overview for Recombinant Fc gamma RII (CD32) antibody (ABIN7542954)

Target: Fc gamma RII (CD32)

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rat

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Stimulation (St), Blocking Peptide (BP)

Clone: AT130-5

Product Details

Purpose: Anti-CD32 [AT130-5], Rat IgG1, kappa

Specificity: This antibody is specific for murine CD32, an Fc gamma surface receptor protein with a low affinity for IgG antibodies that expresses on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. CD32 binds antibody-antigen immune complexes and mediates adaptive immune responses.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c FcgammaRIIB^-/- mice with FcgammaRIIB-rat CD4 domain 3/4 fusion protein.

Isotype: IgG1 kappa

Application Details

Application Notes: The original version of this antibody (mouse IgG1) was used in various FACS, Western blot, and immunofluorescence experiments to demonstrate that, for example, interaction with FcγRIIB is critical for the agonistic activity of anti-CD40 mAb (White et al., 2011, PMID: 21742972), and that immunotherapy targeting murine FcγRIIB is limited by mAb consumption and receptor internalization (Williams et al., 2013, PMID: 24026082). It is noted that, unlike the antagonistic anti-CD32 (clone AT128) antibody, which blocks FcγRIIB receptor phosphorylation, this agonistic anti-CD32 (clone AT130-5) has been shown to activate receptor function while blocking immune complex engagement to the inhibitory FcγRs (Vollack et al., 2017, PMID: 28492697). These differential antagonistic/agonistic anti-CD32 mAbs have been used to probe more specifically the role of CD32 in the murine FVIII-specific recall response (Vollack et al., 2017, PMID: 28492697).

Comment:

This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: Fc gamma RII (CD32)

Alternative Name: CD32

Background: FcγRIIB, FcgRIIb, CD32B, CDw32, FCG2, FCGR2, FCGR2B, IGFR2, Immunoglobulin G Fc receptor II, Low affinity immunoglobulin gamma Fc region receptor II-b, MGC23887, MGC30032, RP11-5K23.6

UniProt: P08101