Recombinant TNFSF4 antibody

Catalog No. ABIN7543010

This Rabbit Chimeric antibody specifically detects TNFSF4 in FACS, IHC (fro) and BP. It exhibits reactivity toward Mouse.

Quick Overview for Recombinant TNFSF4 antibody (ABIN7543010)

Target: TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This TNFSF4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Peptide (BP)

Clone: OX-89

Product Details anti-TNFSF4 Antibody

Purpose: Anti-OX40L [OX-89], Rabbit IgG, kappa

Specificity: This antibody is specific for murine CD252 (the ligand for OX40), expression of which is restricted to activated B cells, dendritic cells, microglia and endothelial cells.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was prepared by immunizing PVG rats with a recombinant chimeric protein consisting of domains 3 and 4 of rat CD4 and the extracellular region of mouse CD134L.

Isotype: IgG kappa

Application Details

Application Notes: This antibody binds mouse CD134L, and has been shown to bind activated B cells and DCs, as well as CD134L-transfected fibroblast cells, though not activated T cells, in labelling and immunohistochemical analysis (Malmstro m, 2001). In addition, this antibody is able to partially block the interaction between CD134 and CD134L. This blocking effect could be responsible for the ability of this antibody to prevent T cell accumulation in the intestine of T cell-restored SCID mice and inhibits the development of Th1-mediated colitis.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TNFSF4

Target: TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))

Alternative Name: OX40L

Background: CD252, Tumor necrosis factor ligand superfamily member 4, OX40 ligand, CD134 ligand, OX40R

UniProt: P43488

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation, Cancer Immune Checkpoints