Recombinant CD53 antibody

Catalog No. ABIN7543047

The Rabbit Chimeric anti-CD53 antibody has been validated for FACS, IP, IHC (fro) and IHC (p). It is suitable to detect CD53 in samples from Rat.

Quick Overview for Recombinant CD53 antibody (ABIN7543047)

Target: CD53

Antibody Type: Recombinant Antibody

Reactivity: Rat

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD53 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: OX-44

Product Details anti-CD53 Antibody

Purpose: Anti-CD53 [OX-44], Rabbit IgG, kappa

Specificity: This antibody recognises CD53, which is expressed on peripheral T-cells, single (CD4 or CD8) positive thymocytes, B-cells, monocytes, macrophages, neutrophils, dendritic cells and microglial cells. This antibody recognises a conformational epitope that is dependent on disulphide bonding.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was prepared by immunizing BALB/c mice with T lymphoblasts from an MLR between purified rat T helper cells and irradiated semiallogeneic rat spleen.

Isotype: IgG kappa

Application Details

Application Notes: This antibody binds rat CD53, and consequently labels all myeloid cells and peripheral lymphoid cells, but only 12 % of thymocytes (Paterson, 1987). Functional studies indicate that this subset of OX-44+ thymic cells includes all thymocytes that are on the pathway to immunocompetent T lymphocytes. By contrast, the OX-44- compartment represents cells that are negative for proliferation and thymopoiesic activity, and are fated to die in situ. In addition, this antibody has been used in conjunction with CD4 and CD8 antigens to define seven subsets of thymic cell. Most CD4-CD8- cells are OX-44+, whereas almost all CD4+ CD8+ cells were OX-44-

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD53

Target: CD53

Alternative Name: CD53

Background: Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Leukocyte antigen MRC OX-44

UniProt: P24485