Recombinant La Crosse Virus,Envelope Glycoprotein 1 (LACV G1) antibody

Catalog No. ABIN7543089

The Rabbit Chimeric anti- antibody has been validated for IP, Neut, ELISA, WB, FACS and Sero. It is suitable to detect in samples from La Crosse Virus (LACV).

Quick Overview for Recombinant La Crosse Virus,Envelope Glycoprotein 1 (LACV G1) antibody (ABIN7543089)

Target: La Crosse Virus,Envelope Glycoprotein 1 (LACV G1)

Antibody Type: Recombinant Antibody

Reactivity: La Crosse Virus (LACV)

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Neutralization (Neut), ELISA, Western Blotting (WB), Flow Cytometry (FACS), Serological Studies (Sero)

Clone: 807-31

Product Details

Purpose: Anti-La Crosse virus, G1 envelope glycoprotein [807.31], Rabbit IgG, kappa

Specificity: This antibody is specific for the Bunyavirus La Crosse G1 viral glycoprotein. According to the original publication (Gonzalez-Scarano et al, 1982), this antibody clone 807.31 was validated to belong to Group A antibodies, which were virus specific and with high neutralization (N) and hemagglutination inhibition (HI) titers. La Crosse Virus is an arbovirus (a virus transmitted by insects), it is one of the most important mosquito-borne viruses in the United States. La Crosse virus contains two glycoproteins, G1 and G2, the larger of which, G1, is the target of neutralizing antibodies.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising BALB/c mice with La Crosse viruses and the harvested spleens were fused with mouse P3X63Ag8.653 myeloma cells.

Isotype: IgG kappa

Application Details

Application Notes: In the original publication, this antibody, together with other 22 clones against the the G1 and N proteins of LaCrosse and Tahyna, was characterised by the ELISA assays, immunoprecipitation, neutralisation tests, and hemagglutination inhibition tests (Gonzalez-Scarano et al, 1982). It was used for antigenic taxonomy of Califmia serogroup viruses and for the identification of the California serogroup viruses of North America (Gonzalez-Scarano et al, 1982). It was also used, together with the other anti-La Crosse mAb clones 807.35 and 807.27, in the neutralisation assays of murine leukemia virus pseudotypes of La Crosse and Hantaan Bunyaviruses to validate a system for analysis of cell tropism (Ma et al, 1999). Furthermore, a single immunisation with this antibody was reported to result in a robust immune response and protection against La Crosse virus (Pekosz et al, 1995). Recently, this antibody has been reported in various FACS analyses, for example, to demonstrate that mutagenesis of the La Crosse Virus glycoprotein supports a role for Gc (1066-1087) as the fusion peptide (Plassmeyer et al, 2007), and to suggest that the fusion peptide of La Crosse virus Gc is a determinant of properties associated with neurotoxicity (Soldan et al, 2010).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: La Crosse Virus,Envelope Glycoprotein 1 (LACV G1)

Alternative Name: La Crosse virus, G1 envelope glycoprotein

Target Type: Viral Protein

Background: LCV G1, G1 envelope glycoprotein, G1 glycoprotein, G1 protein, Envelopment polyprotein, M polyprotein, Glycoprotein G1

UniProt: Q8JPR1