Recombinant La Crosse Virus,Envelope Glycoprotein 1 (LACV G1) antibody
LACV G1
Reactivity: La Crosse Virus (LACV)
IP, Neut, ELISA, WB, FACS, Sero
Host: Rabbit
Chimeric
807-31
unconjugated
Recombinant Antibody
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- Target
- La Crosse Virus,Envelope Glycoprotein 1 (LACV G1)
- Antibody Type
- Recombinant Antibody
- Reactivity
- La Crosse Virus (LACV)
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Host
- Rabbit
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Clonality
- Chimeric
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Conjugate
- Un-conjugated
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Application
- Immunoprecipitation (IP), Neutralization (Neut), ELISA, Western Blotting (WB), Flow Cytometry (FACS), Serological Studies (Sero)
- Purpose
- Anti-La Crosse virus, G1 envelope glycoprotein [807.31], Rabbit IgG, kappa
- Specificity
- This antibody is specific for the Bunyavirus La Crosse G1 viral glycoprotein. According to the original publication (Gonzalez-Scarano et al, 1982), this antibody clone 807.31 was validated to belong to Group A antibodies, which were virus specific and with high neutralization (N) and hemagglutination inhibition (HI) titers. La Crosse Virus is an arbovirus (a virus transmitted by insects), it is one of the most important mosquito-borne viruses in the United States. La Crosse virus contains two glycoproteins, G1 and G2, the larger of which, G1, is the target of neutralizing antibodies.
- Characteristics
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
- Purification
- Protein A affinity purified
- Immunogen
- This antibody was raised by immunising BALB/c mice with La Crosse viruses and the harvested spleens were fused with mouse P3X63Ag8.653 myeloma cells.
- Clone
- 807-31
- Isotype
- IgG kappa
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- Application Notes
- In the original publication, this antibody, together with other 22 clones against the the G1 and N proteins of LaCrosse and Tahyna, was characterised by the ELISA assays, immunoprecipitation, neutralisation tests, and hemagglutination inhibition tests (Gonzalez-Scarano et al, 1982). It was used for antigenic taxonomy of Califmia serogroup viruses and for the identification of the California serogroup viruses of North America (Gonzalez-Scarano et al, 1982). It was also used, together with the other anti-La Crosse mAb clones 807.35 and 807.27, in the neutralisation assays of murine leukemia virus pseudotypes of La Crosse and Hantaan Bunyaviruses to validate a system for analysis of cell tropism (Ma et al, 1999). Furthermore, a single immunisation with this antibody was reported to result in a robust immune response and protection against La Crosse virus (Pekosz et al, 1995). Recently, this antibody has been reported in various FACS analyses, for example, to demonstrate that mutagenesis of the La Crosse Virus glycoprotein supports a role for Gc (1066-1087) as the fusion peptide (Plassmeyer et al, 2007), and to suggest that the fusion peptide of La Crosse virus Gc is a determinant of properties associated with neurotoxicity (Soldan et al, 2010).
- Comment
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Target
- La Crosse Virus,Envelope Glycoprotein 1 (LACV G1)
- Alternative Name
- La Crosse virus, G1 envelope glycoprotein
- Target Type
- Viral Protein
- Background
- LCV G1, G1 envelope glycoprotein, G1 glycoprotein, G1 protein, Envelopment polyprotein, M polyprotein, Glycoprotein G1
- UniProt
- Q8JPR1
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