Recombinant CTNNA1 antibody

Catalog No. ABIN7543127

This Mouse Monoclonal antibody specifically detects CTNNA1 in WB, IF and IP. It exhibits reactivity toward Human.

Quick Overview for Recombinant CTNNA1 antibody (ABIN7543127)

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTNNA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 1G5

Product Details anti-CTNNA1 Antibody

Purpose: Anti-alpha catenin [1G5], Mouse IgG1, kappa

Specificity: This antibody recognizes alpha catenin and binds to the domain between amino acids 135-226. α-catenin associates with the cytoplasmic domain of a variety of plasma membrane spanning cadherins that mediate cell-cell adhesion. The association of catenins to cadherins produces a complex that is linked to the actin filament network, which seems to be of primary importance for their cell-adhesion properties.

Cross-Reactivity: Mouse

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing Balb/c mice with a 90 kDa MBP/alpha catenin fusion protein purified on an amylose column.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody is recommended for the detection and analysis of alpha-catenin via various methods. For instance, it was used to identify alpha-catenin in MDA468 cells via immunofluorescence (Bajpai et al., 2009). It was also repeatedly demonstrated that 1G5 specifically detects alpha-catenin on Western blots (Johnson et al., 1993, Stadler et al., 2018).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CTNNA1

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Alternative Name: alpha catenin

Background: Catenin alpha-1, Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-REN-13, α-catenin, α catenin, α(E)-catenin

UniProt: P35221

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location