Recombinant Desmocollin 2+3 (Extracellular Domain) antibody

Catalog No. ABIN7543132

Product Details

Target: Desmocollin 2+3

Antibody Type: Recombinant Antibody

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purpose: Anti-desmocollin-2/3 [7G6], Rabbit IgG, kappa

Specificity: This antibody recognizes desmocollin-2 (also known as Desmocollin-3). Desmocollins (next to desmogleins) are the main desmosomal transmembrane proteins.The expression of desmocollin-2 varies in the basal and suprabasal layers.

Cross-Reactivity: Mouse, Rat

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing mice with a MBP-tagged recombinant protein corresponding to the extracellular domain of human Desmocollin-2.

Clone: 7G6

Isotype: IgG kappa

Application Details

Application Notes: The antibody 7G6 is recommended for the detection and analysis of desmocollin-2 (desmocollin-3) via various methods, such as immunohistochemistry, Western blot and others. For instance, the antibody was used to label desmocollin-2 in primary human keratinocytes via immunohistochemistry (Franke et al., 2006). Similarly, another group detected desmocollin-2 in A431 cells (Wahl et al., 2005). The antibody 7G6 was also utilized in the assessment of expression levels of desmocollin-2 in SHEEC and KYSE510 cells via Western blot (Fang et al., 2014).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: Desmocollin 2+3

Alternative Name: desmocollin-2/3

Background: Desmocollin, Desmocollin-2, Cadherin family member 2, Desmocollin-3, Desmosomal glycoprotein II, Desmosomal glycoprotein III, Cadherin family member 3, Dsc2/3, Dsc2, Dsc3, DG2, DG3

UniProt: Q02487