Recombinant CEACAM8 antibody

Catalog No. ABIN7543166

This Mouse Monoclonal antibody specifically detects CEACAM8 in FACS, IF, ISG and ISt. It exhibits reactivity toward Human.

Quick Overview for Recombinant CEACAM8 antibody (ABIN7543166)

Target: CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoscintigraphy (ISG), Immunostaining (ISt)

Clone: BW 250-183

Product Details anti-CEACAM8 Antibody

Purpose: Anti-CD66b [BW 250/183], Mouse IgG1, kappa

Specificity: This antibody binds the human CD66b, which is expressed on the surface of granulocytes.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with CEA positive adenocarcinoma cells preferably from lungs.

Isotype: IgG1 kappa

Application Details

Application Notes: This murine antibody reacted with granulocytes, colon mucosa and faintly with pancreatic ducts (PMID: 2410375). The radioactive isotope technetium-99m labelled version of this antibody is also called Besilesomab (trade name Scintimun). It has been studied widely for therapeutic applications. Technetium-99m-Labeled BW 250/183 was used to study the inflamatory process and identification of the inflamatory foci using immunoscintigraphy (PMID: 2157831, 1565572). This antibody is also used for scintiographic imaging of soft tissue infections, peripheral bone, and abdominal infections (PMID: 8023170). Tc-99m-labeled antibody has also been used in the localization of extramedullary hematopoiesis (PMID: 11953571). Rhenium-188-labelled BW250/183 was developed for adjuvant conditioning radioimmunotherapy of bone marrow before bone marrow transplantation. Immunoreactivity of the reduced and rhenium-incorporated antibody was determined by indirect immunofluorescence, by means of a whole blood staining method follwoed by flow ctometric analysis (PMID: 10541824). 90Y-label is reportedled antibody is reported to be used in the study of expression of CD66b in non-Hodgkin lymphomas and multiple myeloma usinf flow cytometry (PMID: 20887387).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CEACAM8

Target: CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))

Alternative Name: CD66b

Background: CEACAM8, CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, Carcinoembryonic antigen CGM6, CD67 antigen, Non-specific cross-reacting antigen, NCA-95

UniProt: P31997