Recombinant KLRK1 antibody

Catalog No. ABIN7543178

This Rat Monoclonal antibody specifically detects KLRK1 in FACS, BP and Neut. It exhibits reactivity toward Mouse.

Quick Overview for Recombinant KLRK1 antibody (ABIN7543178)

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This KLRK1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Peptide (BP), Neutralization (Neut)

Clone: CX-5

Product Details anti-KLRK1 Antibody

Purpose: Anti-NKG2D [CX-5], Rat IgG1, lambda

Specificity: This antibody recognizes mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1 alpha, beta, gamma, delta, epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1 thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original rat IgG1 version of this antibody was raised by immunizing rats against mouse KLRK1.

Isotype: IgG1 lambda

Application Details

Application Notes: It has been shown that CX5 is a non-depleting antibody, which blocks binding of NKG2D to its ligands and mediates internalization of the receptor. This antibody has been widely used to block NKG2D (Wang et al., 2018, pmid: 29447423, Yang et al., 2017, pmid: 28455530) as well as to detect NKG2D-posotive cells via flow cytometry (Hosomi et al., 2017, pmid: 28747426).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for KLRK1

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Alternative Name: NKG2D

Background: CD314, KLRK1, NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D-activating NK receptor, CX5

UniProt: O54709

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process