Recombinant CEA antibody

Catalog No. ABIN7543223

This Mouse Monoclonal antibody specifically detects CEA in IHC, FACS, ELISA, RIA and ISG. It exhibits reactivity toward Human.

Quick Overview for Recombinant CEA antibody (ABIN7543223)

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Radioimmunoassay (RIA), Immunoscintigraphy (ISG)

Clone: T84-66

Product Details anti-CEA Antibody

Purpose: Anti-CEA [T84.66], Mouse IgG1, kappa

Specificity: This antibody binds the A3 domain of the human carcinoembryonic antigen.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing female BALB/c mice subcutaneously with soluble CEA or with the HC84S cell line.

Isotype: IgG1 kappa

Application Details

Application Notes: The binding affinity of the original mouse antibody to carcinoembryonic antigen was determined using a competitive radioimmunoassay (PMID: 6187848). 111In (Indacea) labelled version of this antibody was used to image tumors in patients with colorectal cancer (PMID: 3779658). An engineered antibody fragment (VL-VH-CH3, or "minibody") generated using this antibody having bivalent binding to carcinoembryonic antigen showed high level targeting of xenografts (PMID: 8674062). A radiolabeled chimeric version of this antibody was used to target breast cancer cells expressing high levels of CEA and it was found that chimeric 86.66 resulted in good tumor localization as well as significant tumor growth inhibition. This antibody was also used to detect CEA expression in Human breast carcinoma cell line, MCF 7, using flow cytometry and immunohistochemistry (PMID: 21597719). The characterization of the chimeric version of this antibody generated by transient expression in tobacco leaves was done using ELISA (PMID: 10500141). A phase 1 clinical radioimmunotherapy trial studying 22 patients with metastatic CEA-producing malignancies involving 90Yttrium-labeled chimeric T86.44 revealed that the antibody was well tolerated, with reversible thrombocytopenia and leukopenia being dose limiting (PMID: 11051230). This antibody was used in the immunohistochemical staining of paraffin sections of primary tumours from 252 consecutive patients with breast carcinomas (PMID: 9635845).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CEA

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Alternative Name: CEA

Background: Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CEACAM5, Meconium antigen 100, CD66e

UniProt: P06731