Recombinant CD40 antibody (Extracellular Domain)

Catalog No. ABIN7543233

The Mouse Chimeric anti-CD40 antibody has been validated for FACS, ELISA, IP, IF and Agon. It is suitable to detect CD40 in samples from Mouse.

Quick Overview for Recombinant CD40 antibody (Extracellular Domain) (ABIN7543233)

Target: CD40

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Mouse

Clonality: Chimeric

Conjugate: This CD40 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Agonist (Agon)

Clone: 1C10

Product Details anti-CD40 Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-CD40 [1C10], Mouse IgG1, kappa

Specificity: This antibody binds mouse CD40, which is a member of the tumor necrosis factor receptor superfamily. CD40 is an integral membrane protein found on the surface of B lymphocytes.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing a rat with 20 mug of soluble extracellular domain of recombinant murine CD40 (sCD40).

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody was reported to directly induce a specific proliferative response from mature murine B cells, up-regulate several B cell surface antigens, and rescue immature B lymphoma cells from anti-IgM-induced growth arrest. This initial screening of hybridoma supernatants for reactivity towards CD40 transfected L cells was done using flow cytometry. This antibody recognized recombinant CD40 expressed on L cells and the soluble extracellular domain of murine CD40 coated onto microtiter plates in an ELISA. This antibody was also used in the immunofluorescent staining of adult murine spleen cells. The staining was restricted to the B220+ population. This antibody also specifically immunoprecipitated a 45- kDa protein from the surface of purified murine splenic B cells (PMID: 7519997). This antibody was capable of blocking binding of FGK45 to CD40 on murine B cells in vitro (PMID: 24416732). The mouse IgG1 version of this antibody had a tenfold higher affinity for mouse FcγRIIB as compared to mouse IgG2a or the parental rat IgG2b (PMID: 21852502). The mouse IgG1 version of this antibody was used in the treatment of M38 tumor bearing mice and it was reported that there was a strong increase in PD-L1 expression both on tumor-infiltrating monocytes and TAMs (PMID: 25623164).

Comment:

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background: TNFRSF5, B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, MGC9013, T-cell differentiation antigen, Tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5

UniProt: P27512

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints