Recombinant KRT17 antibody (AA 410-432)

Catalog No. ABIN7543318

This anti-KRT17 antibody is a Rabbit Chimeric antibody detecting KRT17 in IHC and ELISA. Suitable for Human.

Quick Overview for Recombinant KRT17 antibody (AA 410-432) (ABIN7543318)

Target: KRT17 (Keratin 17 (KRT17))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This KRT17 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: 4D2

Product Details anti-KRT17 Antibody

Binding Specificity: AA 410-432

Purpose: Anti-Cytokeratin 17 [4D2], Rabbit IgG, kappa

Specificity: This antibody is specific for amino acids 410-432 (TIVEEVQDGKVISSREQVHQTTR) of human cytokeratin 17. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Cytokeratin 17 regulates the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1).

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing female BALB/c mice with an immunogenic polypeptide containing amino acids 410-432 (TIVEEVQDGKVISSREQVHQTTR) of human cytokeratin 17 conjugated with KLH.

Isotype: IgG kappa

Application Details

Application Notes: The binding characterization of this antibody to human cytokeratin 8 was done using ELISA. The affinity constant for the original IgG3 antibody was calculated to be 9.6 x 10^(8) M^(-1). This antibody is similar to commercially available anti-Cytokeratin 17 antibody E3 in staining various human tissues. This antibody can be used in the immunohistochemical analysis of esophageal squamous cell carcinoma (CN113278070).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for KRT17

Target: KRT17 (Keratin 17 (KRT17))

Alternative Name: Cytokeratin 17

Background: CK17, CK-17, KRT17, K17, Keratin, type I cytoskeletal 17, Cytokeratin-17, Keratin-17, CGMCC NO 20762, E3

UniProt: Q04695