Recombinant Histone H4 antibody

Catalog No. ABIN7543353

The Mouse Monoclonal anti-Histone H4 antibody has been validated for WB, ELISA and Crys. It is suitable to detect Histone H4 in samples from Human.

Quick Overview for Recombinant Histone H4 antibody (ABIN7543353)

Target: Histone H4

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Crystallization (Crys)

Clone: 1A9D7

Product Details anti-Histone H4 Antibody

Purpose: Anti-Histone H4 [1A9D7], Mouse IgG1, kappa

Specificity: The antibody specifically recognizes simultaneous acetylations at K5 and K8 of histone H4. Lysine acetylation of core histones is one of the major posttranslational modifications involved in the control of eukaryotic gene expression.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with Histone H4 protein with site-specific acetylations at K5 and K8.

Isotype: IgG1 kappa

Application Details

Application Notes: The specificity of the original format of the antibody for histone H4 proteins containing di-acetylation of K5acK8ac was confirmed by ELISA analysis. The antibody detected the histone H4 proteins containing at least the di-acetylation of K5acK8ac western blot analysis. The crystal structure of the complex of di-acetylated histone H4 and 1A9D7 Fab fragment was determined (Handoko et al., 2018, PMID: 30080437).

Comment:

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Histone H4

Target: Histone H4

Background: H4K5acK8ac, Histone H4 protein,

UniProt: P62805