Recombinant TIM3 antibody

Catalog No. ABIN7543404

The Rabbit Chimeric anti-TIM3 antibody has been validated for WB, ELISA and IHC. It is suitable to detect TIM3 in samples from Human.

Quick Overview for Recombinant TIM3 antibody (ABIN7543404)

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This TIM3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 7H2

Product Details anti-TIM3 Antibody

Purpose: Anti-TIM-3 [7H2], Rabbit IgG, kappa

Specificity: The original antibody is specific for TIM-3. TIM-3 is a type I transmembrane protein and contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with recombinant TIM-3 protein.

Isotype: IgG kappa

Application Details

Application Notes: The antibody specifically detected TIM-3 protein in Western blot analysis. The antibody 7H2 and the commercial antibody D5D5R were used to stain tonsils and appendix tissue. Results showed the antibody specificity is comparable to the commercial antibody (CN113234158A).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TIM3

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Alternative Name: TIM-3

Background: CD366, Hepatitis A virus cellular receptor 2, Hepatitis A virus cellular receptor 2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3, TIMD-3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, D5D5R, E3, CGMCCNO 20763

UniProt: Q8TDQ0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints