Nestin antibody (AA 681-750) (Cy5.5)

Catalog No. ABIN723535

This Rabbit Polyclonal antibody specifically detects Nestin in FACS, IF (p) and IF (cc). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Nestin antibody (AA 681-750) (Cy5.5) (ABIN723535)

Target: Nestin (NES)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is conjugated to Cy5.5

Application: Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-Nestin Antibody (Cy5.5)

Binding Specificity: AA 681-750

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat Nestin

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background:

Synonyms: ESTM 46, FLJ 21841, FLJ21841, Intermediate filament protein, Nbla00170, Nes, NEST_RAT, Nestin.

Background: Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Gene ID: 25491

UniProt: P21263