ASRGL1 antibody (HRP)

Catalog No. ABIN7565725

This Goat Polyclonal antibody specifically detects ASRGL1 in WB, IHC, ELISA and DB. It exhibits reactivity toward Cow.

Quick Overview for ASRGL1 antibody (HRP) (ABIN7565725)

Target: ASRGL1 (Asparaginase Like 1 (ASRGL1))

Reactivity: Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This ASRGL1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Dot Blot (DB)

Product Details anti-ASRGL1 Antibody (HRP)

Purpose: Alkaline Phosphatase Antibody HRP Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].

Characteristics: Synonyms: goat anti-Alkaline Phosphatase Antibody HRP Conjugation, Peroxidase Conjugation goat anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

Purification: Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Alkaline Phosphatase [Calf Intestine]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Alkaline Phosphatase peroxidase has been tested by dot blot and is functional in ELISA, Western blot, and Immunohistochemical assays. Optimization should be reached by researcher. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for ASRGL1

Target: ASRGL1 (Asparaginase Like 1 (ASRGL1))

Alternative Name: ALP1

Background: Background: Alkaline Phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. Humans and most other mammals contain the following alkaline phosphatase isozymes: ALPI - intestinal, ALPL - tissue non-specific (liver/bone/kidney), ALPP - placental (Regan isozyme). Elevated ALP indicates that there could be active bone formation occurring as ALP is a byproduct of osteoblast activity (such as the case in Paget's disease of bone). Levels are also elevated in people with untreated Coeliac Disease. All mammalian alkaline phosphatase isoenzymes except placental (PALP and SEAP) are inhibited by homoarginine, and, in similar manner, all except the intestinal and placental ones are blocked by levamisole. Heating for ~2 hours at 65 °C inactivated most isoenzymes except Placental isoforms (PALP and SEAP).

Gene ID: 280993

UniProt: P19111