HBG1 antibody (N-Term)

Catalog No. ABIN7565772

This anti-HBG1 antibody is a Mouse Monoclonal antibody detecting HBG1 in ELISA and WB. Suitable for Human.

Quick Overview for HBG1 antibody (N-Term) (ABIN7565772)

Target: HBG1 (Hemoglobin, gamma A (HBG1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HBG1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Clone: 4B3-B5-F3-B7

Product Details anti-HBG1 Antibody

Binding Specificity: N-Term

Purpose: Hemoglobin beta F Antibody

Cross-Reactivity (Details): This protein A purified mouse monoclonal antibody reacts specifically with human HbF gamma isoform.

Characteristics: Synonyms: mouse anti-HbF antibody, mouse anti-hemoglobin antibody, Gamma-1-globin, Hb F Agamma, Hemoglobin gamma-1 chain, Hemoglobin gamma-A chain, HBG1, HBG2, HbF Antibody, Sickle Cell Disease (SCD)

Purification: Anti-HbF is purified from tissue culture supernatant by protein A purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Hemoglobin beta F Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Application Note: Anti-Hemoglobin beta F (MOUSE) antibody has been tested ELISA and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa. Western Blot Dilution: 1 μg/mL ELISA Dilution: 1:20,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for HBG1

Target: HBG1 (Hemoglobin, gamma A (HBG1))

Alternative Name: HBG1

Background: Background: HbF antibodies detect the hemoglobin gamma isoform subunit. Functional alternate hemoglobin (Hb) is a hetero tetramer composed of 2 alpha and 2 gamma subunits (alpha-2 gamma-2). Hemoglobin F is elevated in newborns, reaching adult levels by 12 months. HbF levels are increased to as much as 5 % to 10 % in normal pregnancy. Sickle cell disease (SCD), thalassemias and hemoglobinopathies occur when aberrant forms of hemoglobin are expressed in children and adults. Hemoglobin variants arise from mutations in the globin genes and sickle cell disease and the more benign sickle cell trait are observed in more than 100 million people globally. HbF antibody does not react other forms of Hb. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.

Gene ID: 3047

NCBI Accession: NP_000550

UniProt: P69891