LYZ antibody (HRP)

Catalog No. ABIN7565788

This Rabbit Polyclonal antibody specifically detects LYZ in WB, IHC, IP and ELISA. It exhibits reactivity toward Chicken.

Quick Overview for LYZ antibody (HRP) (ABIN7565788)

Target: LYZ (Lysozyme (LYZ))

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYZ antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA

Product Details anti-LYZ Antibody (HRP)

Purpose: Lysozyme Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lysozyme [Hen Egg White].

Characteristics: Synonyms: rabbit anti-Lysozyme Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Lysozyme Antibody, 1 4 beta n acetylmuramidase c antibody, EC 3.2.1.17 antibody, LYZ antibody, Lysozyme C antibody, Lysozyme C precursor antibody, LZM antibody, Hen Egg White

Purification: Anti-Lysozyme Antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Lysozyme Antibody was produced by repeated immunizations with hen egg white Lysozyme protein.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Lysozyme Peroxidase Conjugate Antibody has been tested by western blotting and ELISA and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for LYZ

Target: LYZ (Lysozyme (LYZ))

Alternative Name: LYZ

Background: Background: Anti-Lysozyme Antibody detects lysozyme. Lysozyme belongs to the glycoside hydrolases enzymes that damage bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. Lysozyme is abundant in a number of secretions, such as tears, saliva, human milk, and mucus. It is also present in cytoplasmic granules of the polymorphonuclear neutrophils. Large amounts of lysozyme can be found in egg white. Anti-Lysozyme Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.

Gene ID: 396218

NCBI Accession: NP_990612

UniProt: P00698