Protein A antibody (FITC)

Catalog No. ABIN7565825

The Goat Polyclonal anti-Protein A antibody has been validated for WB, DB and FACS. It is suitable to detect Protein A in samples from Staphylococcus aureus.

Quick Overview for Protein A antibody (FITC) (ABIN7565825)

Target: Protein A

Reactivity: Staphylococcus aureus

Host: Goat

Clonality: Polyclonal

Conjugate: This Protein A antibody is conjugated to FITC

Application: Western Blotting (WB), Dot Blot (DB), Flow Cytometry (FACS)

Product Details anti-Protein A Antibody (FITC)

Purpose: Protein A Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, and purified and partially purified Protein A.

Characteristics: Synonyms: goat anti-Protein A Antibody, FITC Conjugated goat anti-Protein A Antibody, Protein A Fluorescein, Protein A FITC Antibody

Purification: Anti-Protein A Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Protein A (Staphylococcus aureus)

Immunogen Type: Native Protein

Labeling Ratio: 4.3

Application Details

Application Notes: Application Note: Anti-Protein A has been tested in dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Protein A

Target: Protein A

Background: Background: Protein A is 56 kDA surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG and others and is therefore suitable for purification and detection of immunoglobulins.