TET2 antibody (N-Term)

Catalog No. ABIN7565848

This Rabbit Polyclonal antibody specifically detects TET2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for TET2 antibody (N-Term) (ABIN7565848)

Target: TET2 (Tet Methylcytosine Dioxygenase 2 (TET2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TET2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TET2 Antibody

Binding Specificity: N-Term

Purpose: TET2 Antibody

Characteristics: Synonyms: rabbit anti-TET2 Antibody, Tet2 antibody, Methylcytosine dioxygenase TET2, KIAA1546 antibody, myeloproliferative disorder antibody, MPD antibody, hematopoietic cell antibody, TET2 antibody, Tet oncogene 2 antibody, MDS antibody, TET2_HUMAN antibody

Purification: Anti-TET2 Antibody was prepared from whole rabbit antiserum by delipidation and defibrination. The antiserum was further cross-absorbed against MBP by chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: TET2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a human TET-2 domain containing the N-terminal 156 aminoacids of the protein.

Immunogen Type: Recombinant Protein

Application Details

Application Notes: Application Note: Tet2 antibody has been tested by Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect band at ~230 kDa and ~150 kDa (isoforms). This antibody is suitable for use by ELISA. Western Blot Dilution: 1:5000 ELISA Dilution: 1:50,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 72.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for TET2

Target: TET2 (Tet Methylcytosine Dioxygenase 2 (TET2))

Alternative Name: TET2

Background: Background: Tet2 antibody is a dioxygenase enzyme which is associated with the addition of the hydroxyl group to 5-methylcystosine (5mC) to form 5-hydroxymethylcystosine. Tet2 mediates the formation of 5-formylcystosine from 5hmC and subsequently 5fC to 5-carboxylcystosine, and consequently Tet2 plays an active role in catalyzing those conversions which have been suggested as the first step for active mammalian DNA de-methylation. Tet2 enzyme aids in the epigenetic modification of mammalian cystosine bases, ultimately affecting transcriptional regulation. Additionally methylcystosine dioxygenase Tet2 recruits O-GlcNAc transferase OGT enzyme to CpG-rich transcription start sites of the gene, encouraging H2B GlcNAcylation by OGT. Anti-TET2 antibodies are ideal for researchers interested in Epigenetics, Cancer, Chromatin Research and Histone research.

Gene ID: 54790

NCBI Accession: NP_001120680

UniProt: Q6N021

Pathways: Warburg Effect