CGA antibody

Catalog No. ABIN7565947

This Goat Polyclonal antibody specifically detects CGA in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CGA antibody (ABIN7565947)

Target: CGA (Glycoprotein Hormones, alpha Polypeptide (CGA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CGA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CGA Antibody

Purpose: hCG Alpha chain Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against hCG alpha.

Characteristics: Synonyms: Goat Anti-Human Chorionic Gonadotropin Alpha Antibody, Goat anti-hCGa Antibody, α-hCG, a-hCG, alpha-hCG, Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, CG-alpha, Follicle-stimulating hormone alpha chain, FSH-alpha, Follitropin alpha chain, Luteinizing hormone alpha chain, LSH-alpha, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, TSH-alpha, Thyrotropin alpha chain

Purification: This affinity purified antibody is directed against hCG alpha.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Human Chorionic Gonadotropin Alpha Antibody was prepared from whole goat serum produced by repeated immunizations with Hu Chorionic Gonadotropin Alpha Subunit protein.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Human Chorionic Gonadotropin (hCG) Alpha Antibody has been tested in ELISA and WB.  Expect a band at ~14.9 kDa for the alpha chain in western blot using appropriate tissues and lysates. ELISA reactivity expected against alpha hCG with an expected titer of ~1:100,000 and full hCG at ~1:50,000 dilution. Western Blot Dilution: 1:1000 ELISA Dilution: 1:20,000 - 1:100,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CGA

Target: CGA (Glycoprotein Hormones, alpha Polypeptide (CGA))

Alternative Name: CGA

Background: Background: Human Chorionic Gonadotropin (hCG) is a member of the glycoprotein hormone family which includes lutropin, follitropin, and thyrotropin. These hormones are heterodimers consisting of a common alpha subunit, and a unique beta chain which confers their biological specificity to the hormones. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways. HCG is secreted in large quantities by normal trophoblasts. Its presence is in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. CGA is extracellular and secreted, expressed in over 90 organs, but has its highest expression level in placenta. Anti-hCG alpha detects hCG alpha in human cells and tissue and could be useful in detecting cells and tumors of trophoblastic origin such as choriocarcinoma. Anti-hCG Antibody is useful for researchers interested in pregnancy, hormone research, and cancer research.

Gene ID: 1081

NCBI Accession: NP_000726

UniProt: P01215

Pathways: Metabolism of Steroid Hormones and Vitamin D, Thyroid Hormone Synthesis, Hormone Transport, Peptide Hormone Metabolism