Transferrin antibody (FITC)

Catalog No. ABIN7565982

This anti-Transferrin antibody is a Rabbit Polyclonal antibody detecting Transferrin in WB, ELISA, DB, FM and FLISA. Suitable for Human.

Quick Overview for Transferrin antibody (FITC) (ABIN7565982)

Target: Transferrin (TF)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transferrin antibody is conjugated to FITC

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Fluorescence Microscopy (FM), FLISA

Product Details anti-Transferrin Antibody (FITC)

Purpose: Transferrin Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and purified and partially purified Transferrin (Human Serum).

Characteristics: Synonyms: rabbit anti-Transferrin Antibody Fluorescein Conjugated, Apotransferrin antibody, Beta 1 metal binding globulin antibody, DKFZp781D0156 antibody, PRO1400 antibody, PRO1557 antibody, PRO2086 antibody, Serotransferrin precursor antibody, Siderophilin antibody, TF antibody

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Transferrin (Human Serum)

Immunogen Type: Native Protein

Labeling Ratio: 4.5

Application Details

Application Notes: Application Note: Anti-Human transferrin Fluorescein has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Western Blot Dilution: User Optimized FLISA Dilution: User Optimized ELISA Dilution: 1:10,000 - 1:20,000 IF Microscopy Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Transferrin

Target: Transferrin (TF)

Alternative Name: Transferrin

Background: Background: Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality. Human Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.

Gene ID: 7018

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis