Recombinant Human Immunodeficiency Virus Transmembrane Glycoprotein (HIV gp41) antibody
Quick Overview for Recombinant Human Immunodeficiency Virus Transmembrane Glycoprotein (HIV gp41) antibody (ABIN7566426)
Target
Antibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- anti-gp41 (HIV), mAb (rec.) (A2)
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Characteristics
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Recombinant Antibody. Recognizes gp41 from HIV. Targets the pocket-binding domain of gp41 CHR when CHRs maintain in the trimer conformation. Applications: ELISA, FACS, IP. Clone: A2. Isotype: Human IgG1. Formulation: Liquid. In PBS. HIV-1 Env consists of two subunits: the surface-exposed glycoprotein gp120, which contains the receptor (CD4) and coreceptor (CCR5 or CXCR4) binding sites, and the transmembrane subunit gp41, which is critical for virus-cell membrane fusion. Sequential binding of HIV-1 to its receptor and coreceptor are the first steps critical for viral entry, whereby CD4 binding triggers a series of conformational changes in Env involving both gp120 and gp41. In gp120, CD4 binding induces rearrangement of the variable loops V1/V2 and formation of the bridging sheet, which allows for repositioning of the V3 loop to facilitate coreceptor binding. In gp41, CD4 binding triggers exposure of the buried N-terminal HR1 domain and its formation into a trimeric coiled-coil structure. Upon receptor and coreceptor binding, the fusion peptide in gp41 is inserted into the cell membrane and the HR1 and HR2 regions rearrange to form the six-helix bundle, which is critical for creation of the fusion pore to enable membrane fusion and efficient viral entry. The antibody clone A2 was found to specifically target the pocket-binding domain of gp41 CHR when CHRs maintain in the trimer conformation.
HIV-1 Env consists of two subunits: the surface-exposed glycoprotein gp120, which contains the receptor (CD4) and coreceptor (CCR5 or CXCR4) binding sites, and the transmembrane subunit gp41, which is critical for virus-cell membrane fusion. Sequential binding of HIV-1 to its receptor and coreceptor are the first steps critical for viral entry, whereby CD4 binding triggers a series of conformational changes in Env involving both gp120 and gp41. In gp120, CD4 binding induces rearrangement of the variable loops V1/V2 and formation of the bridging sheet, which allows for repositioning of the V3 loop to facilitate coreceptor binding. In gp41, CD4 binding triggers exposure of the buried N-terminal HR1 domain and its formation into a trimeric coiled-coil structure. Upon receptor and coreceptor binding, the fusion peptide in gp41 is inserted into the cell membrane and the HR1 and HR2 regions rearrange to form the six-helix bundle, which is critical for creation of the fusion pore to enable membrane fusion and efficient viral entry. The antibody clone A2 was found to specifically target the pocket-binding domain of gp41 CHR when CHRs maintain in the trimer conformation.
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Purification
- Puified
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Purity
- >95 % (SDS-PAGE)
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Immunogen
- Lectin-purified gp140 (from ADA.C1 strain).
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Isotype
- IgG1
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- In PBS.
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Handling Advice
- After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.Please handle under sterile conditions to avoid contamination.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Stable for at least 1 year after receipt when stored at -20°C.
Stable for at least 1 week when stored at +4°C.
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- Human Immunodeficiency Virus Transmembrane Glycoprotein (HIV gp41)
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Alternative Name
- gp41
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Target Type
- Viral Protein
Target
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