Recombinant Human Immunodeficiency Virus Transmembrane Glycoprotein (HIV gp41) antibody
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- Target
- Human Immunodeficiency Virus Transmembrane Glycoprotein (HIV gp41)
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human Immunodeficiency Virus (HIV)
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Host
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Human
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)
- Purpose
- anti-gp41 (HIV), mAb (rec.) (A2)
- Characteristics
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Recombinant Antibody. Recognizes gp41 from HIV. Targets the pocket-binding domain of gp41 CHR when CHRs maintain in the trimer conformation. Applications: ELISA, FACS, IP. Clone: A2. Isotype: Human IgG1. Formulation: Liquid. In PBS. HIV-1 Env consists of two subunits: the surface-exposed glycoprotein gp120, which contains the receptor (CD4) and coreceptor (CCR5 or CXCR4) binding sites, and the transmembrane subunit gp41, which is critical for virus-cell membrane fusion. Sequential binding of HIV-1 to its receptor and coreceptor are the first steps critical for viral entry, whereby CD4 binding triggers a series of conformational changes in Env involving both gp120 and gp41. In gp120, CD4 binding induces rearrangement of the variable loops V1/V2 and formation of the bridging sheet, which allows for repositioning of the V3 loop to facilitate coreceptor binding. In gp41, CD4 binding triggers exposure of the buried N-terminal HR1 domain and its formation into a trimeric coiled-coil structure. Upon receptor and coreceptor binding, the fusion peptide in gp41 is inserted into the cell membrane and the HR1 and HR2 regions rearrange to form the six-helix bundle, which is critical for creation of the fusion pore to enable membrane fusion and efficient viral entry. The antibody clone A2 was found to specifically target the pocket-binding domain of gp41 CHR when CHRs maintain in the trimer conformation.
HIV-1 Env consists of two subunits: the surface-exposed glycoprotein gp120, which contains the receptor (CD4) and coreceptor (CCR5 or CXCR4) binding sites, and the transmembrane subunit gp41, which is critical for virus-cell membrane fusion. Sequential binding of HIV-1 to its receptor and coreceptor are the first steps critical for viral entry, whereby CD4 binding triggers a series of conformational changes in Env involving both gp120 and gp41. In gp120, CD4 binding induces rearrangement of the variable loops V1/V2 and formation of the bridging sheet, which allows for repositioning of the V3 loop to facilitate coreceptor binding. In gp41, CD4 binding triggers exposure of the buried N-terminal HR1 domain and its formation into a trimeric coiled-coil structure. Upon receptor and coreceptor binding, the fusion peptide in gp41 is inserted into the cell membrane and the HR1 and HR2 regions rearrange to form the six-helix bundle, which is critical for creation of the fusion pore to enable membrane fusion and efficient viral entry. The antibody clone A2 was found to specifically target the pocket-binding domain of gp41 CHR when CHRs maintain in the trimer conformation.
- Purification
- Puified
- Purity
- >95 % (SDS-PAGE)
- Immunogen
- Lectin-purified gp140 (from ADA.C1 strain).
- Clone
- A2
- Isotype
- IgG1
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- In PBS.
- Handling Advice
- After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.Please handle under sterile conditions to avoid contamination.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Stable for at least 1 year after receipt when stored at -20°C.
Stable for at least 1 week when stored at +4°C.
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- Target
- Human Immunodeficiency Virus Transmembrane Glycoprotein (HIV gp41)
- Alternative Name
- gp41
- Target Type
- Viral Protein
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