ESR2 antibody (AA 201-300)

Catalog No. ABIN725210

The Rabbit Polyclonal anti-ESR2 antibody has been validated for WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect ESR2 in samples from Human, Mouse, Rat and Sheep. There are 2+ publications available.

Quick Overview for ESR2 antibody (AA 201-300) (ABIN725210)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivity: Human, Mouse, Rat, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESR2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ESR2 Antibody

Binding Specificity: AA 201-300

Cross-Reactivity: Human, Mouse, Rat, Sheep

Predicted Reactivity: Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ER-beta

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ESR2 antibody (ABIN725210)

Wei, Guo, Mu, Zhang, Zhao, Huai, Qiu, Li, Ma, Liu, Chen, Han: "Estrogen suppresses hepatocellular carcinoma cells through ERβ-mediated upregulation of the NLRP3 inflammasome." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 95, Issue 7, pp. 804-16, (2015) (PubMed).

Fang, Wu, Huang: "Raloxifene upregulated mesangial cell MMP-2 activity via ER-? through transcriptional regulation." in: Cell biochemistry and biophysics, Vol. 67, Issue 2, pp. 607-13, (2013) (PubMed).

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: Estrogen receptor beta

Background:

Synonyms: Erb, ESRB, ESTRB, NR3A2, ER-BETA, ESR-BETA, Estrogen receptor beta, Nuclear receptor subfamily 3 group A member 2, ESR2

Background: Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

Gene ID: 2100

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling