HSP70 antibody (AA 481-534)

Catalog No. ABIN725360

The Rabbit Polyclonal anti-HSP70 antibody has been validated for WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect HSP70 in samples from Human, Mouse, Rat and Chicken. There are 3+ publications available.

Quick Overview for HSP70 antibody (AA 481-534) (ABIN725360)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Human, Mouse, Rat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HSP70 Antibody

Binding Specificity: AA 481-534

Cross-Reactivity: Chicken, Human, Mouse, Rat

Predicted Reactivity: Dog,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HSP70

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-HSP70 antibody (ABIN725360)

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

Wang, Wang, Feng, Mi, Chen, Shang, Chen: "Comparative vesicle proteomics reveals selective regulation of protein expression in chestnut blight fungus by a hypovirus." in: Journal of proteomics, (2012) (PubMed).

Wang, Ma, Yang, Zhao, Yao, Jian, Luo, Xia: "Proteomic analysis of the sea-island cotton roots infected by wilt pathogen Verticillium dahliae." in: Proteomics, Vol. 11, Issue 22, pp. 4296-309, (2011) (PubMed).

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: HSP70

Background:

Synonyms: HSP72, HSPA1, HSP7I, HSP7-1, HSP7-1A, HEL-S-13, Heat shock 7 kDa protein 1A/1B, Heat shock 7 kDa protein 1/2, HSP7-1/HSP7-2, HSP7.1/HSP7.2, HSPA1A, HSX7

Background: In cooperation with other chaperones, Hsp7s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.

Gene ID: 3303

UniProt: P08107