VAC14 antibody (AA 523-782)

Catalog No. ABIN7581221

Product Details anti-VAC14 Antibody

Target: VAC14 (Vac14 Homolog (VAC14))

Binding Specificity: AA 523-782

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAC14 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Rabbit polyclonal antibody to human Vac14 with reactivity to mouse/rat and human Vac14

Specificity: The Vac14 antibody is directed at an C-terminal region of Vac14 and detects mammalian forms of Vac14 with an expected molecular weight of approximately 82-88 kDa.

Cross-Reactivity: Mouse, Rat

Purification: affinity purified using Protein A chromatography

Immunogen: The antibody was generated from a fusion protein with amino acids 523 to 782 of human Vac14.

Isotype: IgG

Application Details

Application Notes: IF/ICC – 2-5 ug/mL, Western – 1.0 ug/mL, Immunoprecipitation – 1.0 ug/mL Other in vitro and cellular applications are possible using this antibody, but have not been verified.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4

Storage: 4 °C,-80 °C

Storage Comment: protect from direct light and store at 4°C for up to 30 days. For long term storage, aliquot and store at -80°C. Avoid repeated freeze/thaw cycles.

Target Details for VAC14

Target: VAC14 (Vac14 Homolog (VAC14))

Alternative Name: Vac14 (VAC14 Products)

Background: Vac14 is a scaffold protein that functions in a complex with PIKfyve and Sac3 (FIG4) to regulate the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) on endosomal membranes. The coordinated activity within the complex maintains homeostasis of PI(3)P and PI(5)P and plays a role in the fusion and transport of endosomes

Pathways: Inositol Metabolic Process