GFAP antibody (AA 51-150)

Catalog No. ABIN726200

The Rabbit Polyclonal anti-GFAP antibody has been validated for WB, ELISA, FACS, ICC, IHC (p), IHC (fro), IF (cc) and IF (p). It is suitable to detect GFAP in samples from Human, Rat and Mouse. There are 20+ publications available.

Quick Overview for GFAP antibody (AA 51-150) (ABIN726200)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-GFAP Antibody

Binding Specificity: AA 51-150

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GFAP

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-GFAP antibody (ABIN726200)

Farah, Siwek, Cummings: "Tau accumulations in the brains of woodpeckers." in: PLoS ONE, Vol. 13, Issue 2, pp. e0191526, (2018) (PubMed).

Liao, Xiao, Zhu, Zhou: "The effect of celastrol on learning and memory in diabetic rats after sevoflurane inhalation." in: Archives of medical science : AMS, Vol. 14, Issue 2, pp. 370-380, (2018) (PubMed).

Hu, Kodithuwakku, Zhou, Li, Han, Fang, Liu, Li: "Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 Pathway." in: Molecules (Basel, Switzerland), Vol. 22, Issue 6, (2017) (PubMed).

Sweeney, Qi, Xu, Yang: "Activation of hypothalamic astrocytes suppresses feeding without altering emotional states." in: Glia, Vol. 64, Issue 12, pp. 2263-2273, (2016) (PubMed).

Yan, Li, Li, Lin, Yang: "Osthole Protects Bone Marrow-Derived Neural Stem Cells from Oxidative Damage through PI3K/Akt-1 Pathway." in: Neurochemical research, (2016) (PubMed).

Zhao, Sun, Miao, Ji, Zhao, Sun, Miao, Yoshii, Mizushima, Wang, Zhang: "The autophagy gene Wdr45/Wipi4 regulates learning and memory function and axonal homeostasis." in: Autophagy, Vol. 11, Issue 6, pp. 881-90, (2015) (PubMed).

Mori, Matsubara, Matsubara, Uchikura, Hashimoto, Fujioka, Matsumoto: "Stromal Cell-Derived Factor-1? Plays a Crucial Role Based on Neuroprotective Role in Neonatal Brain Injury in Rats." in: International journal of molecular sciences, Vol. 16, Issue 8, pp. 18018-32, (2015) (PubMed).

Shan, Huang, You, Yuan, Gao, Hu, Du: "High efficiency intracellular transport of cationic peptide stearate for gene delivery in tumor cells and multipotent stem cells." in: Journal of biomedical nanotechnology, Vol. 10, Issue 11, pp. 3231-43, (2015) (PubMed).

Liang, Sun, Yu, He, Xie: "Scorpion ethanol extract and valproic acid effects on hippocampal glial fibrillary acidic protein expression in a rat model of chronic-kindling epilepsy induced by lithium chloride-pilocarpine." in: Neural regeneration research, Vol. 7, Issue 6, pp. 426-33, (2015) (PubMed).

Fan, Hu, Ji, Sun, Xiong, Yang, Liu: "Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons." in: In vitro cellular & developmental biology. Animal, Vol. 50, Issue 4, pp. 304-12, (2014) (PubMed).

Xiang, Wei, Chen, Liu, Liu, Wang, Zhang: "Anti-inflammatory effect of acetylpuerarin on eicosanoid signaling pathway in primary rat astrocytes." in: Journal of molecular neuroscience : MN, Vol. 52, Issue 4, pp. 577-85, (2014) (PubMed).

Ma, Cao, Li, Gao, Qi, Zhao, Du, Xue, Peng, Wen, Chen, Ning, Huang, Zhang, Gao, Yu, Chen: "Dapper1 promotes autophagy by enhancing the Beclin1-Vps34-Atg14L complex formation." in: Cell research, Vol. 24, Issue 8, pp. 912-24, (2014) (PubMed).

Du, Liu, Chen, Dou, Lei, Chang, Cai, Cui, Yang, Sun, Li, Jiang: "Targeting the SMO oncogene by miR-326 inhibits glioma biological behaviors and stemness." in: Neuro-oncology, (2014) (PubMed).

Zuo, Wang, Li, Lin, Duan, Qi, Li, Sun, Wu: "Existence of glia mitigated ketamine-induced neurotoxicity in neuron-glia mixed cultures of neonatal rat cortex and the glia-mediated protective effect of 2-PMPA." in: Neurotoxicology, Vol. 44, pp. 218-30, (2014) (PubMed).

Hu, Zheng, Jia, Li, Dong, Wang, Lin, Zhang, Zhao, Xu, Zhou, Yuan, Zhang, Ren: "Neuroprotection effect of interleukin (IL)-17 secreted by reactive astrocytes is emerged from a high-level IL-17-containing environment during acute neuroinflammation." in: Clinical and experimental immunology, Vol. 175, Issue 2, pp. 268-84, (2014) (PubMed).

Zhao, Zhao, Wang, Xu, Miao, Feng, Chen, Kovács, Fan, Zhang: "Mice deficient in Epg5 exhibit selective neuronal vulnerability to degeneration." in: The Journal of cell biology, Vol. 200, Issue 6, pp. 731-41, (2013) (PubMed).

Liu, Xue, Tang, Hou, Qi, Chen, Chen, Zhang, Chen, Xu: "A simple method for isolating and culturing the rat brain microvascular endothelial cells." in: Microvascular research, Vol. 90, pp. 199-205, (2013) (PubMed).

Xiang, Chen, Liu, Liu, Wei, Sun, Wang, Zhang: "Inhibition of sPLA?-IIA prevents LPS-induced neuroinflammation by suppressing ERK1/2-cPLA?? pathway in mice cerebral cortex." in: PLoS ONE, Vol. 8, Issue 10, pp. e77909, (2013) (PubMed).

Zhao, Zhao, Miao, Wang, Sun, Zhang: "The p53-induced gene Ei24 is an essential component of the basal autophagy pathway." in: The Journal of biological chemistry, Vol. 287, Issue 50, pp. 42053-63, (2012) (PubMed).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background:

Synonyms: Glial fibrillary acidic protein, GFAP

Background: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Gene ID: 2670

UniProt: P14136