CHRNB1 antibody (Extracellular)

Catalog No. ABIN7581859

This Rabbit Polyclonal antibody specifically detects CHRNB1 in WB. It exhibits reactivity toward Rat.

Quick Overview for CHRNB1 antibody (Extracellular) (ABIN7581859)

Target: CHRNB1 (Nicotinic Acetylcholine Receptor beta (CHRNB1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHRNB1 Antibody

Binding Specificity: AA 68-80, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β1

Sequence: (C)EKDEEMSTKV YLD

Specificity: Extracellular, N-terminus

Predicted Reactivity: Mouse,human - identical

Characteristics: Highly specific antibody directed against an epitope of rat nAChR β1. Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody (ABIN7581859) can be used in western blot analysis. As the antibody recognizes an extracellular epitope, it can be used to detect the receptor in living cells. It has been designed to nAChRβ1 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EKDEEMSTKVYLD, corresponding to amino acid residues 68-80 of rat nAChRbeta1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CHRNB1

Target: CHRNB1 (Nicotinic Acetylcholine Receptor beta (CHRNB1))

Alternative Name: CHRNB1

Background: NAChR β1, ACHRB,Neuronal nicotinic acetylcholine receptors (nAChRs) belong to the superfamily of ligand-gated ion channels and are widely expressed throughout the central and peripheral nervous systems. nAChRs play crucial roles in modulating a wide range of higher cognitive functions by mediating presynaptic, postsynaptic, and extrasynaptic signaling1.nAChRs are formed by the assembly of five transmembrane subunits, selected from a pool of 17 homologous polypeptides (α1-10, β1-4, γ, δ, and ε). There are many nAChR subtypes, each consisting of a specific combination of subunits, which mediate diverse physiological functions. They are widely expressed in the central nervous system, while, in the periphery, they mediate synaptic transmission at the neuromuscular junction and ganglia. nAChRs are also found in non-neuronal/non-muscle cells (keratinocytes, epithelia, macrophages, etc.)2. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, three conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore3.While most β subunits are neuronal, the β1 subunit forms functional receptors along with other subunits in the muscle2. β1 subunit seems to be involved in myasthenia gravis (MG)4, an acquired autoimmune disease usually characterized by the presence of circulating autoantibodies that bind to and destroy muscle nAChRs5.

Gene ID: 24261

UniProt: P25109

Pathways: Synaptic Membrane