CNTN2 antibody (Extracellular)

Catalog No. ABIN7581864

This anti-CNTN2 antibody is a Rabbit Polyclonal antibody detecting CNTN2 in WB and IHC. Suitable for Mouse.

Quick Overview for CNTN2 antibody (Extracellular) (ABIN7581864)

Target: CNTN2 (Contactin 2 (Axonal) (CNTN2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNTN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CNTN2 Antibody

Binding Specificity: AA 76-90, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Contactin-2 (extracellular)

Sequence: (C)KMNGTEMNLE PGSRH

Specificity: Extracellular, N-terminus

Predicted Reactivity: Rat,human - 14 out of 15 amino acid residues identical

Characteristics: Anti-Contactin-2/CNTN2 (extracellular) Antibody (ABIN7581864) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Contactin-2 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KMNGTEMNLEPGSRH, corresponding to amino acid residues 76 - 90 of mouse CNTN2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CNTN2

Target: CNTN2 (Contactin 2 (Axonal) (CNTN2))

Alternative Name: CNTN2 

Background: Axonal glycoprotein TAG-1, Axonin-1, Transient axonal glycoprotein 1, TAX-1,Contactin-2 (also known as Axonal glycoprotein TAG-1, Axonin-1, Transient axonal glycoprotein 1, TAX-1, CNTN2) is a cell adhesion molecule that belongs to the immunoglobulin superfamily (IgSF).IgSF proteins mediate protein-protein interactions. They are either secreted or localized to the cell surface. Many of the cell surface IgSF proteins are adhesion molecules (IgCAMs) that mediate interactions among neurons, and between neurons and glia.1The immunoglobulin superfamily is divided into subgroups, one of which includes the contactin subfamily that consists of six members (Contactin-1 to Contactin-6). All six molecules of the contactin subgroup have six extracellular immunoglobulin-like domains and four fibronectin III-like domains, are anchored to the lipid bilayer of the cell membrane via glycosylphosphatidylinositol (GPI), and are found on the membrane surface.2 Contactin-2 is primarily expressed on the axonal and synaptic membranes. It is expressed in hippocampal pyramidal cells, cerebellar granule cells, the juxtaparanodal regions of myelinated nerve fibers, and frontal and temporal lobes. Contactin-2 is a multifunctional protein, in the embryonic nervous system, it plays important roles in axonal elongation, axonal guidance, and cellular migration. In the postnatal nervous system, it plays a role in neuronal fasciculation, axonal domain organization, formation of myelinated nerve fibers, and neuron-glia interaction.3Contactin-2 interacts with contactin associated protein-like 2 (caspr2) at the juxtaparanodal region of nodes of Ranvier. This association is necessary for the maintenance of voltage-gated potassium channels (VGKCs) at juxtaparanodal regions of myelinated axons.Caspr2 autoantibodies disrupt the binding of Caspr2 to contactin-2, resulting in hyperexcitability of peripheral nerves. These autoantibodies have been associated with a form of acquired peripheral nerve hyperexcitability (acquired neuromyotonia) and autoimmune encephalitis. 4Contactin-2 is also a target protein of autoantibodies in patients with Multiple Sclerosis (MS)5. It was identified as one of the elevated cerebrospinal fluid proteins in a proteomics study in pediatric MS patients.6Contactin-2 interacts with proteins involved in alzheimer's disease (AD) pathogenesis, such as amyloid precursor protein (APP) and beta-secretase 1 (BACE1). These interactions may influence the production of Aβ peptide and the subsequent formation of amyloid plaques. Single nucleotide polymorphisms (SNPs) in the gene encoding contactin-2 (CNTN2) are associated with AD. In addition, higher levels of contactin-2 have been reported in AD CSF pools using proteomics approaches.3In addition, Contactin-2 is reported to be overexpressed in malignant gliomas, where it is involved in the growth and differentiation of glioma cells. It is also associated with development of oligodendrogliomas.2

Gene ID: 21367

UniProt: Q61330

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size