GAP43 antibody (AA 205-218)

Catalog No. ABIN7581879

This anti-GAP43 antibody is a Guinea Pig Polyclonal antibody detecting GAP43 in WB and IHC. Suitable for Rat.

Quick Overview for GAP43 antibody (AA 205-218) (ABIN7581879)

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This GAP43 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GAP43 Antibody

Binding Specificity: AA 205-218

Purpose: A Guinea Pig Polyclonal Antibody to GAP43

Sequence: (C)KPKESARQDE GKED

Specificity: C-terminus

Predicted Reactivity: Mouse - identical, human - 13 out of 14 amino acid residues identical

Characteristics: Guinea Pig Anti-GAP43 Antibody (ABIN7581879) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GAP43 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-GAP43 Antibody (ABIN7043263, ABIN7044662 and ABIN7044663) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KPKESARQDEGKED, corresponding to amino acid residues 205-218 of rat GAP43

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235735)

Blocking Peptide: (ABIN7235735)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Alternative Name: GAP43

Background: Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50,Growth associated protein 43 (GAP43) is a neuron specific phosphoprotein that plays an important role in the process of nerve growth, development, regeneration and axonal remodeling. GAP43 belongs to the calmodulin-binding protein family and is highly distributed in the presynaptic membrane1.GAP43 is expressed in developing neurons, especially in the growth cone. In mature neurons, GAP43 is detected in regenerating nerves. In brain injury, neurons exhibit sprouting of the lateral branches and responsive regeneration of the axon accompanied by increased expression of GAP43. GAP43 expression is used as an important tool for evaluating axon injury and the regenerative response in the mature central nervous system (CNS)1,2.

Gene ID: 29423

UniProt: P07936