GFRAL antibody (Extracellular)

Catalog No. ABIN7581881

The Rabbit Polyclonal anti-GFRAL antibody has been validated for WB and IHC. It is suitable to detect GFRAL in samples from Rat.

Quick Overview for GFRAL antibody (Extracellular) (ABIN7581881)

Target: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFRAL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GFRAL Antibody

Binding Specificity: AA 320-334, Extracellular

Purpose: A Rabbit Polyclonal antibody to GFRAL

Sequence: (C)YPTPNVKDIS SYERK

Specificity: Extracellular, N-terminus

Predicted Reactivity: Mouse - 14 out of 15 amino acid residues identical It will not recognize the human protein

Characteristics: Anti-GFRAL (extracellular) Antibody (ABIN7581881) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GFRAL from rat and mouse samples. The antibody will not recognize human GFRAL.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)YPTPNVKDISSYERK, corresponding to amino acid residues 320 - 334 of rat GFRAL 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:400

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GFRAL

Target: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Alternative Name: GFRAL

Background: GDNF Family Receptor Alpha-Like,GFRAL, also known as GDNF Family Receptor Alpha-Like, is an orphan member of the GFR-α family. GFRAL is a brainstem-restricted receptor for Growth differentiation factor 15 (GDF15), which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Upon interaction with its ligand GDF15, GFRAL interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.GFRAL regulates body weight and the receptor mediating the metabolic effects of GDF15, enabling potential pharmacotherapies for the treatment of obesity. GFRAL is expressed in neurons of the area postrema and the nucleus of the solitary tract in mice and humans, and genetic deletion of the receptor abrogates the ability of GDF15 to decrease food intake and body weight in mice. Moreover, diet-induced obesity and insulin resistance are exacerbated in GFRAL-deficient mice, suggesting a homeostatic role for this receptor in metabolism1.GDF15, a distant member of the transforming growth factor (TGF)-β family, is a secreted protein that circulates in plasma as a 25- kDa homodimer. In humans, elevated GDF15 correlates with weight loss, and the administration of GDF15 to mice with obesity reduces body weight, at least in part, by decreasing food intake.GDF15 has been implicated in various biological functions, including cancer cachexia, renal and heart failure, atherosclerosis, and metabolism. A connection between GDF15 and body-weight regulation was initially suggested based on an observation that increasing GDF15 levels in serum correlated with weight loss in individuals with advanced prostate cancer. GFRAL-/- mice were refractory to the effects of recombinant human GDF15 on body weight, food intake and glucose parameters. Blocking the interaction between GDF15 and GFRAL with a monoclonal antibody prevented the metabolic effects of GDF15 in rats. GFRAL mRNA is highly expressed in the area postrema of mouse, rat and monkey models, in accordance with previous reports implicating this region of the brain in the metabolic actions of GDF152.The GDNF family includes glial cell line-derived neurotrophic factor (GDNF), artemin (ARTN), Neurturin (NRTN) and persephin (PSPN)3. These factors are heavily involved in the development and function of the nervous system (both central and peripheral). GDNF serves an important role outside the nervous system, where it plays a role in kidney morphogenesis4.In general, GFLs all signal through the receptor tyrosine kinase RET. Their specificity is implemented by different GDNF family receptors a (GFRα), which act as co-receptors. These extracellular proteins are bound to the plasma membrane via a glycosyl phosphatidyl inositol (GPI) anchor. GFRα1-4 are responsible for the binding of GDNF, NRTN, ARTN, and PSPN respectively and the subsequent activation of RET. Soluble forms of the receptor, produced by the cleavage of a yet unknown phospholipase or protease, can be detected. Alternative spliced forms of the protein can also lead to the production of soluble forms of GFRα receptors5.

Gene ID: 501023

UniProt: D3ZB94