GPR158 antibody (Intracellular)

Catalog No. ABIN7581886

This Rabbit Polyclonal antibody specifically detects GPR158 in WB and IHC. It exhibits reactivity toward Mouse.

Quick Overview for GPR158 antibody (Intracellular) (ABIN7581886)

Target: GPR158 (G Protein-Coupled Receptor 158 (GPR158))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR158 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GPR158 Antibody

Binding Specificity: AA 960-974, Intracellular

Purpose: A Rabbit Polyclonal antibody to GPR158 receptor

Sequence: (C)EPRKPQKSGI MKQQR

Specificity: Intracellular, C-terminus.

Predicted Reactivity: Human - identical,Rat - 14 out of 15 amino acid residues identical

Characteristics: Anti-GPR158 Antibody (ABIN7581886) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR158 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EPRKPQKSGIMKQQR, corresponding to amino acid residues 960 - 974 of mouse GPR158

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR158

Target: GPR158 (G Protein-Coupled Receptor 158 (GPR158))

Alternative Name: GPR158

Background: Probable G-protein coupled receptor 158, Metabotropic Glycine Receptor, mGlyR,G protein-coupled receptor 158 (GPR158) is a protein that belongs to the G protein-coupled receptor (GPCR) superfamily. GPR158 was identified as an orphan receptor and a member of Class C GPCRs. 1Class C GPCRs are obligatory dimers and are characterized by a large N- terminus extracellular ligand-binding domain linked to the seven-transmembrane (7TM) domain, and a large intracellular C-terminus domain. Members of Class C GPCRs include metabotropic glutamate receptors (mGluRs) and GABA(B) receptors, among others.1,2GPR158 is highly expressed in the central nervous system (CNS), particularly in the hippocampus, cortex, midbrain, and cerebellum. Indeed, GPR158 has been linked to affective disorders, such as memory loss, cognitive diseases and depression. 2Recently, osteocalcin (OCN) has been proposed as a putative ligand for GPR158. OCN is is a protein primarily produced by osteoblasts and appears to mediate its memory and cognitive regulation properties, through GPR158.3,4Lately however, GPR158 was identified as the long sought metabotropic receptor mediating the slow neuromodulatory effects of glycine (Gly) and in analogy to the well-known metabotropic glutamate receptors (mGluRs), it has been named as mGlyR.5,6The recognition that GPR158 function is central for cognition and affective states, may pave the way for the development of novel antidepressant drugs targeting GPR158.Finally, GPR158 expression has been found to be elevated in several types of cancer including glioma, prostate and ovarian cancinomas.2

Gene ID: 241263

UniProt: Q8C419

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling