GPR173 antibody (Intracellular)

Catalog No. ABIN7581888

This anti-GPR173 antibody is a Rabbit Polyclonal antibody detecting GPR173 in WB and IHC. Suitable for Mouse.

Quick Overview for GPR173 antibody (Intracellular) (ABIN7581888)

Target: GPR173 (G Protein-Coupled Receptor 173 (GPR173))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR173 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GPR173 Antibody

Binding Specificity: AA 274-286, Intracellular

Purpose: A Rabbit Polyclonal Antibody to GPR173

Sequence: (C)GMDEVKGEKQ LGR

Specificity: Intracellular, 3rd loop.

Cross-Reactivity (Details): This antibody is specific for GPR173, it will not recognize the highly homologous receptor GPR85 (SREB2).

Predicted Reactivity: Rat,human - identical.

Characteristics: Anti-GPR173 Antibody (ABIN7581888) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR173 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)GMDEVKGEKQLGR, corresponding to amino acid residues 274 - 286 of mouse GPR173

Isotype: IgG

Application Details

Application Notes: Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR173

Target: GPR173 (G Protein-Coupled Receptor 173 (GPR173))

Alternative Name: GPR173

Background: G-Protein Coupled Receptor 173, Super Conserved Receptor Expressed In Brain 3, SREB3,GPR173, also referred to as SREB3 (Super Conserved Receptor Expressed in Brain 3), is a G-protein-coupled receptor (GPCR) belonging to the superfamily of Class A GPCRs. GPR173 is primarily localized to the cell membrane and is prominently expressed in the brain and gonads, particularly within hypothalamic kisspeptin and gonadotropin-releasing hormone (GnRH) neurons1,2. The receptor is activated by Phoenixin (PNX), a neuropeptide involved in reproductive and hormonal regulation, as well as by GnRH-(1-5). PNX binding to GPR173 triggers the cAMP/PKA and MAPK signaling pathways, leading to increased expression of genes regulating reproduction, such as GnRH and Kiss1. On the other hand, GnRH-(1-5) activates GPR173 via the β-arrestin pathway, demonstrating its capacity for biased agonism2,3. Beyond reproduction, GPR173 plays a significant role in modulating inflammatory and oxidative stress pathways. Inflammatory stimuli such as lipopolysaccharide (LPS) reduce GPR173 expression in astrocytes, microglia, and dental pulp cells. Activation of GPR173 by PNX-14 or PNX-20 has been shown to suppress pro-inflammatory cytokines and chemokines, as well as reduce oxidative stress by decreasing ROS production and increasing antioxidant enzymes such as superoxide dismutase (SOD)3,4,6,7. GPR173 also attenuates LPS-induced endoplasmic reticulum (ER) stress by inhibiting the eIF-2α/ATF4/CHOP signaling pathway, preventing the activation of the NLRP3 inflammasome and the subsequent production of IL-1β and IL-18. These protective effects extend to reducing mitochondrial dysfunction, cell death, and infarct volume in neuroinflammatory and ischemic models3,4,7,8. In vivo studies with siRNA knockdown of Gpr173 have shown significant disruptions in estrous cycling and reproductive hormone signaling, confirming its essential role in maintaining reproductive function. Dysregulation of GPR173 has also been linked to neuroinflammation, oxidative stress, and cell death, suggesting potential therapeutic implications for neurodegenerative diseases, stroke, and inflammatory disorders4,7,8.

Gene ID: 70771

UniProt: Q6PI62