HCRTR1 antibody (Intracellular)

Catalog No. ABIN7581897

This anti-HCRTR1 antibody is a Guinea Pig Polyclonal antibody detecting HCRTR1 in WB and IHC. Suitable for Rat.

Quick Overview for HCRTR1 antibody (Intracellular) (ABIN7581897)

Target: HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This HCRTR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HCRTR1 Antibody

Binding Specificity: AA 256-269, Intracellular

Purpose: A Guinea Pig Polyclonal Antibody to Orexin Receptor 1

Sequence: (C)RNWKRPSEQL EAQH

Specificity: 3rd intracellular loop

Predicted Reactivity: Mouse - identical,human - 9 out of 14 amino acid residues identical

Characteristics: Guinea Pig Anti-Orexin Receptor 1 Antibody (ABIN7581897) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Orexin Receptor 1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Orexin Receptor 1 Antibody (ABIN7043285, ABIN7044883 and ABIN7044884) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 of rat OX1R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235802)

Blocking Peptide: (ABIN7235802)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HCRTR1

Target: HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))

Alternative Name: HCRTR1

Background: OX1R, Hypocretin receptor type 1, HCRTR1,Orexin Receptor 1 (OX1R) (also known as hypocretin receptor 1) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference), while OX2R binds both ligands with similar affinities.2,3Both OX1R and OX2R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.OX1R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels.2,3The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research in the last few years.OX1R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2 It appears to be involved in the regulation of feeding behavior in rodents since an OX1R antagonist is able to inhibit baseline feeding.2,3The orexin system has been shown to be involved in the regulation of sleep and wakefulness states and OX1R knockout mice show defects in the regulation of rapid eye movement (REM) sleep, among other phenotypic alterations.4 In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4

Gene ID: 25593

UniProt: P56718

Pathways: Feeding Behaviour