L1CAM antibody (Extracellular)

Catalog No. ABIN7581912

This anti-L1CAM antibody is a Rabbit Polyclonal antibody detecting L1CAM in WB, IHC, FACS and LCI. Suitable for Human.

Quick Overview for L1CAM antibody (Extracellular) (ABIN7581912)

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This L1CAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-L1CAM Antibody

Binding Specificity: AA 653-667, Extracellular

Purpose: A Rabbit Polyclonal antibody to L1CAM (extracellular)

Sequence: (C)EDKEMAPEKW YSLGK

Specificity: Extracellular, N-terminus.

Predicted Reactivity: Mouse,rat - 14 out of 15 amino acid residues identical

Characteristics: Anti-L1CAM (extracellular) Antibody (ABIN7581912) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize L1CAM from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EDKEMAPEKWYSLGK, corresponding to amino acid residues 653 - 667 of human L1CAM

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for L1CAM

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Alternative Name: L1CAM

Background: L1 Cell Adhesion Molecule, Neural Cell Adhesion Molecule L1, NCAM-L1, CAML1, CD171,L1 cell adhesion molecule (L1CAM), also known as neural cell adhesion molecule L1 (NCAM-L1) and CD171, is a transmembrane glycoprotein of the immunoglobulin (Ig) superfamily involved in neuronal structure and function and cell adhesion dynamics.1L1CAM is composed of 1257 amino acids comprising six Ig-like domains, five fibronectin type III repeats, a transmembrane region, and a highly conserved cytoplasmic tail. L1CAM is the prototype member of the L1-family of closely related neural cell adhesion molecules (CAMs). It has been shown that L1CAM is regulated by the Wnt/β-catenin signaling pathway, which is involved in maintaining cellular homeostasis, and nuclear β-catenin co-localizes with L1CAM staining in tumor sections. It has also been shown that in endometrial carcinomas, L1CAM is regulated by two distinct promoter regions and that SLUG, a zinc-finger transcription factor, is important for this regulation.1L1CAM plays a crucial role in neuronal migration, axon growth, and synapse formation in the developing nervous system. Dysfunction of L1CAM leads to clinically variable neurological disorders that are jointly referred to as L1 syndrome, including hydrocephalus, mental disabilities, aphasia, spastic paraplegia, and adducted thumbs.2Although L1CAM was originally identified as a neuronal cell adhesion molecule, it has also been shown to be an essential component for disseminated cancer cells from breast, lung, kidney, and colorectal carcinomas to initiate proliferation in the brain, lung, liver, and bone. L1CAM is used to adhere and spread on the surface of blood capillaries and to activate mechanotransduction-sensitive transcription factors, which are required for the initiation of metastatic outgrowth in perivascular sites.3L1CAM has been found in exosomes and extracellular vesicles in various contexts. For example, in the context of cancer, L1CAM-positive exosomes have been implicated in promoting cancer cell migration and invasion. Exosomes carrying L1CAM can interact with other cells, such as immune cells or endothelial cells, and potentially promote tumor progression and metastasis 4,5.Furthermore, in the nervous system, L1CAM is expressed in neurons and plays a role in axon guidance and neural cell adhesion. Exosomes containing L1CAM may be involved in interneuronal communication and potentially influence neural development and plasticity 4,5.

Gene ID: 3897

UniProt: P32004

Pathways: Synaptic Membrane