MEGF10 antibody (Extracellular)

Catalog No. ABIN7581914

The Rabbit Polyclonal anti-MEGF10 antibody has been validated for WB, IHC, FACS and LCI. It is suitable to detect MEGF10 in samples from Mouse.

Quick Overview for MEGF10 antibody (Extracellular) (ABIN7581914)

Target: MEGF10 (Multiple EGF-Like-Domains 10 (MEGF10))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEGF10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-MEGF10 Antibody

Binding Specificity: AA 178-190, Extracellular

Purpose: A Rabbit Polyclonal antibody to MEGF10 (extracellular)

Sequence: CEDR(S)EQGTYGND

Specificity: Extracellular, N-terminus.

Cross-Reactivity (Details): This antibody is specific for MEGF10, it won't recognize the closely related protein MEGF11 (Accession Q80T91)

Predicted Reactivity: Mouse,rat,human - 12 out of 13 amino acid residues identical

Characteristics: Anti-MEGF10 (extracellular) Antibody (ABIN7581914) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize MEGF10 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CEDR(S)EQGTYGND, corresponding to amino acid residues 178 - 190 of mouse MEGF10

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for MEGF10

Target: MEGF10 (Multiple EGF-Like-Domains 10 (MEGF10))

Alternative Name: MEGF10

Background: Multiple EGF Like Domains 10, Multiple EGF-Like Domains Protein 10,MEGF10 is a member of the multiple epidermal growth factor-like domains protein family. MEGF10 is a type I transmembrane protein that consists of 15 EGF-like domains in its extracellular region and is the mammalian homolog of Draper (a Drosophila phagocytosis apoptotic cell receptor)1.MEGF10 is a mediator for apoptotic cell phagocytosis by central nervous system (CNS) macrophages- microglia and astrocytes, through binding with high affinity to C1q, an eat-me signal, that binds phosphatidylserine expressed on the surface of apoptotic cells2. Also necessary for astrocyte-dependent apoptotic neuron clearance in the developing cerebellum2. In this regard, MEGF10 works alongside other phagocytic receptors expressed in astrocytes or microglia such as the tyrosine kinase receptors MERTK and AXL, the G-protein coupled receptors Bai1 and GPR56 or the TREM2 receptor3.MEGF10 is involved in the uptake of amyloid-β peptide (Aβ42) in the brain. Alzheimer's disease (AD) is characterized by extracellular accumulation of senile plaques, the major component of which is the amyloid-β peptide (Aβ), and the presence of neurofibrillary tangles. MEGF10 as a functional receptor that mediates the uptake of amyloid-β peptide will help elucidate the molecular mechanisms of amlyoid-β clearance in Alzheimer's disease1,4.MEGF10 is also an essential factor in the regulation of myogenesis. It controls the balance between skeletal muscle satellite cells proliferation and differentiation through regulation of the notch signaling pathway5. In fact, mutations in the MEGF10 gene are the underlying cause of Early-onset myopathy, areflexia, respiratory distress, and dysphagia (EMARDD), a lethal congenital myopathy characterized by hypotonia with respiratory distress 5,6.

Gene ID: 70417

UniProt: Q6DIB5

Pathways: Regulation of Muscle Cell Differentiation