PTGER2 antibody (Extracellular)

Catalog No. ABIN7581947

The Rabbit Polyclonal anti-PTGER2 antibody has been validated for WB, IHC, IF, FACS and LCI. It is suitable to detect PTGER2 in samples from Rat.

Quick Overview for PTGER2 antibody (Extracellular) (ABIN7581947)

Target: PTGER2 (Prostaglandin E Receptor 2 (Subtype EP2), 53kDa (PTGER2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTGER2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-PTGER2 Antibody

Binding Specificity: AA 287-299, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Prostanoid EP2 Receptor

Sequence: (C)DETSSRKEKW DLR

Specificity: 3rd extracellular loop

Predicted Reactivity: Mouse - 12,13 amino acid residues identical, human - 11

Characteristics: Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (ABIN7581947) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize EP2 receptor from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DETSSRKEKWDLR, corresponding to amino acid residues 287-299 of rat prostanoid EP2 receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PTGER2

Target: PTGER2 (Prostaglandin E Receptor 2 (Subtype EP2), 53kDa (PTGER2))

Alternative Name: PTGER2

Background: Prostaglandin E2 receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor,Prostaglandin E2 (PGE2) is involved in a number of physiological and pathophysiological events in many tissues throughout the body1. The physiological actions of PGE2 are mediated through its interaction with cell surface prostaglandin E receptors.There are three pharmacologically defined subtypes of the EP receptor, EP1, EP2, and EP3, and these subtypes are suggested to be different in their signal transduction2. These receptors belong to the G-protein coupled receptor (GPCR) superfamily. Like all members they have seven transmembrane domains with an extracellular N-terminal tail and an intracellular C-terminus. The EP2 receptor is expressed in the vasculature, the gastrointestinal tract, kidney and also in the ciliary muscles of the eye3.PGE2 is known to play a central role in the pathophysiology of inflammation in synergy with other proinflammatory mediators. PGE2 inhibits the function and the proliferation of T cells and the histamine release from mast cells by increasing the intracellular level of cAMP4.The EP2 subtype is thought to be in part responsible for vasodilation, oedema formation, hyperanalgesia, modulation of the immune system, and the breakdown of bone and cartilage associated with disorders such as rheumatoid arthritis5.

Gene ID: 81752

UniProt: Q62928