Enolase antibody

Catalog No. ABIN7595318

Product Details anti-Enolase Antibody

Target: Enolase

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This Enolase antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Chicken antibody to Enolase

Specificity: Specific for Enolase (alpha beta gamma).

Cross-Reactivity: Human, Marmoset, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: Purified IgY

Immunogen: A synthetic peptide from AA of 10-50 of human Enolase conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.

Isotype: IgY

Application Details

Application Notes: WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8C for a shorter term.

Expiry Date: 12 months

Target Details for Enolase

Target: Enolase

Abstract: Enolase Products

Background: Function: Multifunctional enzyme that as well as its role in glycolysis plays a part in various processes such as growth control hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. Mammalian enolase is composed of 3 isozyme subunits alpha beta and gamma which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding. In vitro interacts with several glycolytic enzymes including PKM2 PGM CKM and aldolase. Also binds troponin in vitro. Subcellular location: Cytoplasm. Cell membrane. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M-band. Tissue specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. In striated muscle expression of ENO1 appears to be independent of fiber type.