SARS-CoV-2 Spike antibody (AA 671-688)

Catalog No. ABIN7595334

The Guinea Pig Polyclonal anti-SARS-CoV-2 Spike antibody has been validated for WB and IHC. It is suitable to detect SARS-CoV-2 Spike in samples from SARS Coronavirus-2 (SARS-CoV-2).

Quick Overview for SARS-CoV-2 Spike antibody (AA 671-688) (ABIN7595334)

Target: SARS-CoV-2 Spike

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This SARS-CoV-2 Spike antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SARS-CoV-2 Spike Antibody

Binding Specificity: AA 671-688

Purpose: Guinea pig antibody to SARS-Cov-2 spike polybasic region (671-688)

Specificity: Specific for SARS-Cov-2 spike glycoprotein polybasic region

Cross-Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Purification: Whole serum

Immunogen: Synthetic peptide "ASYQTQTNSPRRARSVA" aa 671-688 of SARS spike glycoprotein polybasic region conjugated to blue carrier protein was used as the antigen.

Application Details

Application Notes: IHC WB. A dilution of 1: 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for SARS-CoV-2 Spike

Target: SARS-CoV-2 Spike

Background: Function: Spike protein S1:attaches the virion to the cell membrane by interacting with host receptor initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. The stalk domain of S contains three hinges giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model the protein has at least three conformational states: pre-fusion native state pre-hairpin intermediate state and post-fusion hairpin state. During viral and target cell membrane fusion the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis. Spike glycoprotein: May down-regulate host tetherin (BST2) by lysosomal degradation thereby counteracting its antiviral activity.