SHANK1 antibody (AA 469-691) (FL490)

Catalog No. ABIN7595454

The Mouse Monoclonal anti-SHANK1 antibody has been validated for IHC and ICC. It is suitable to detect SHANK1 in samples from Rat.

Quick Overview for SHANK1 antibody (AA 469-691) (FL490) (ABIN7595454)

Target: SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHANK1 antibody is conjugated to FL490

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: N22-21

Product Details anti-SHANK1 Antibody (FL490)

Binding Specificity: AA 469-691

Purpose: Anti-Shank1 Antibody FL490 Conjugate

Specificity: No off-targets reported for Shank2 or Shank3

Cross-Reactivity: Human, Mouse, Rat

Characteristics:

Description: Our Anti-Shank1 mouse monoclonal primary antibody is produced in-house from hybridoma clone N22/21. It detects human, mouse, and rat Shank1, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Manufacturer Comment: We produce our Shank1 mouse monoclonal primary antibody from hybridoma clone N22/21. It is great in IHC, ICC and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb.

Purity: > 90 % specific antibody

Immunogen: Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1 (accession number Q9WV48) produced recombinantly in E. Coli

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.09 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 12 months

Target Details for SHANK1

Target: SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))

Alternative Name: Shank1

Background:

Synonyms: SH3 and multiple ankyrin repeat domains protein 1 (Shank1) (GKAP/SAPAP-interacting protein) (SPANK-1) (Somatostatin receptor-interacting protein) (SSTR-interacting protein) (SSTRIP) (Synamon)

Target Description: SH3 and multiple ankyrin repeat domains protein 1, or shank 1 is a member of the shank family. Shank 1 binding domains include a SH3 domain, a proline rich domain, a PDZ domain and ankyrin repeat domains. Unlike other shank family proteins, shank 1 is found in only in brain in the neuropil of cortex, CA1 region hippocampus and molecular layer of cerebellum, amygdala, substantia nigra and thalamus. Cellularly, shank 1 can be found in cytoplasm, cell junctions, postsynaptic cell membrane and postsynaptic density. Many isoforms of shank 1 exist, and some are expressed during development, while others are expressed in mature tissue. Shank 1 acts as a scaffold protein anchoring and connecting membrane bound receptors and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 1 may play a role in synapse formation and dendritic spine maturation. Mutations in this gene have been associated with autism spectrm disorder and diabetic encephalopathy.

Gene Name Alternatives: Shank1

Molecular Weight: 190-220 kDa (alternative splice variants)

Pathways: Synaptic Membrane, Maintenance of Protein Location