SHANK2 antibody (AA 84-309) (FL550)

Catalog No. ABIN7595603

This Mouse Monoclonal antibody specifically detects SHANK2 in IHC and ICC. It exhibits reactivity toward Rat.

Quick Overview for SHANK2 antibody (AA 84-309) (FL550) (ABIN7595603)

Target: SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHANK2 antibody is conjugated to FL550

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: N23B-6

Product Details anti-SHANK2 Antibody (FL550)

Binding Specificity: AA 84-309

Purpose: Anti-Shank2 Antibody FL550 Conjugate

Specificity: No off-targets reported for Shank1 or Shank3

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Characteristics:

Description: Our Anti-Shank2 mouse monoclonal primary antibody is produced in-house from hybridoma clone N23B/6. It detects human, mouse, and rat Shank2, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Manufacturer Comment: We produce our Shank2 mouse monoclonal primary antibody from hybridoma clone N23B/6. It is great in IHC, ICC and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb.

Purity: > 90 % specific antibody

Immunogen: Fusion protein amino acids 84-309 (SH3/PDZ domains) of rat Shank2 (accession number Q9QX74) produced recombinantly in E. Coli

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.09 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 12 months

Target Details for SHANK2

Target: SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))

Alternative Name: Shank2

Background:

Synonyms: SH3 and multiple ankyrin repeat domains protein 2 (Shank2) (Cortactin-binding protein 1) (CortBP1) (GKAP/SAPAP-interacting protein) (Proline-rich synapse-associated protein 1) (ProSAP1) (SPANK-3)

Target Description: SH3 and multiple ankyrin repeat domains protein 2, or shank 2 is a member of the shank family. Shank 2 binding domains include a SH3 domain, a proline rich domain, and a PDZ domain. Shank 2 is found in kidney, liver tissue, and epithelial cells. In brain, Shank 2 is expressed at the apical cell membrane, cytoplasm, synapse, postsynaptic density, growth cone, and dendritic spine. Some isoforms of shank 2 are expressed during development, while others are expressed in mature tissue. Shank 2 acts as a scaffold protein anchoring and connecting membrane bound receptors, including NMDA and glutamate receptors and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 2 may play a role in synapse formation and dendritic spine maturation. Mutations in the Shank 2 gene have been associated with autism spectrm disorder and blount disease.

Gene Name Alternatives: Shank2 Cortbp1

Molecular Weight: 160 kDa