TRIM antibody (Intracellular) (Biotin)

Catalog No. ABIN7596010

The Mouse Monoclonal anti-TRIM antibody has been validated for WB, IP and ICFC. It is suitable to detect TRIM in samples from Human.

Quick Overview for TRIM antibody (Intracellular) (Biotin) (ABIN7596010)

Target: TRIM (TRAT1) (T Cell Receptor Associated Transmembrane Adaptor 1 (TRAT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRIM antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC)

Clone: TRIM-04

Product Details anti-TRIM Antibody (Biotin)

Binding Specificity: AA 29-186, Intracellular

Purpose: Anti-TRIM Biotin

Specificity: The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.

No Cross-Reactivity: Mouse

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen: Recombinant intracellular domain (aa 29-186) of human TRIM.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for TRIM

Target: TRIM (TRAT1) (T Cell Receptor Associated Transmembrane Adaptor 1 (TRAT1))

Alternative Name: TRIM

Background: T cell receptor associated transmembrane adaptor 1,TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.,TRAT, TCRIM

Gene ID: 50852

UniProt: Q6PIZ9

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway