CD73 antibody (AA 27-252)

Catalog No. ABIN7596058

The Mouse Monoclonal anti-CD73 antibody has been validated for WB, FACS and ELISA. It is suitable to detect CD73 in samples from Human.

Quick Overview for CD73 antibody (AA 27-252) (ABIN7596058)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Clone: AT89E9

Product Details anti-CD73 Antibody

Binding Specificity: AA 27-252

Purpose: Human 5'-Nucleotidase/CD73 antibody

Purification: protein-A affinity chromatography

Immunogen: Recombinant human 5'-Nucleotidase/CD73 (27-252aa) purified from E. coli

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycero

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: 5'-Nucleotidase/CD73

Background: CD73 (Ecto-5-nucleotidase, 5-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.

NCBI Accession: NP_002517

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process